ChAT (Choline Acetyltransferase) Antibody | Rockland Immunochemicals 600-101-D16 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

ChAT (Choline Acetyltransferase) Antibody

catalog :

600-101-D16

quantity :

100 µL

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse, chicken

application :

western blot, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

citations: 1

Reference
Liu K, Kim J, Kim D, Zhang Y, Bao H, Denaxa M, et al. Lhx6-positive GABA-releasing neurons of the zona incerta promote sleep. Nature. 2017;548:582-587 pubmed publisher

product information

Catalog Number :

600-101-D16

Name :

Anti-ChAT (Choline Acetyltransferase) (Goat) Antibody - 600-101-D16

Display Name :

ChAT (Choline Acetyltransferase) Antibody

Application Note :

Anti-ChAT antibody is suitable for use in ELISA, Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a ~ 68/70 kDa band corresponding to choline acetyltransferase protein by Western Blot in the appropriate lysate or tissue.

Buffer :

0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Clonality :

Polyclonal

Concentration Value :

Titrated value sufficient to run approximately 10 mini blots.

Conjugation :

(None)

Size :

100 µL

Default Unit :

Immunohistochemistry Dilution :

1:100

Western Blot Dilution :

1:100

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

CHAT

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-ChAT Antibody was produced in rabbit by repeated immunizations with native choline acetyltransferase purified from human placenta.

Packing Type :

Dry Ice

Physical State :

Liquid

Preservative :

0.02% (w/v) Sodium Azide

Purity and Specificity :

Anti-ChAT antibody is directed against human ChAT protein. The antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Cross reactivity with ChAT from other species has not been determined. However, reactivity is also expected from the following species based on 100% sequence homology: chicken, guinea pig, mouse, and non-human primates.

Species Reactivity :

Avian, Chicken, Guinea Pig, Human, Mouse, non-Human primates

Stabilizer :

5mg BSA

Storage :

Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Synonyms :

acetyl CoA:choline O-acetyltransferase, CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O-acetyltransferase, CLAT, CMS1A, CMS1A2

Target Species :

Human

Background :

ChAT antibody recognizes choline acetyltransferase which is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. ChAT is found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome. Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects. It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function. Anti-ChAT antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Immunogen Type :

Native Protein

Low Endotoxin :

Sample Size :

Application Text :

Immunohistochemistry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P28329

Primary Image Name :

Western Blot of Anti-Choline Acetyltransferase Antibody

more info or order :

Rockland Immunochemicals product webpage