BMI1 Antibody | Rockland Immunochemicals 600-101-392 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

BMI1 Antibody

catalog :

600-101-392

quantity :

100 µg

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-101-392

Name :

Anti-BMI1 (GOAT) Antibody - 600-101-392

Display Name :

BMI1 Antibody

Application Note :

This affinity purified antibody has been tested for use in ELISA and western blot. A 1:200 dilution is reported to be effective for Immunofluorescence against Methanol fixed cells. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Bmi1 by western blotting in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

0.91 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:5,000 - 1:30,000

IF Microscopy Dilution :

1:200

Western Blot Dilution :

1:500 - 1:3,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

BMI1

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 252-264 of human Bmi1 protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against human Bmi1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Bmi1 protein from human, rat, orangutan, dog, chimpanzee, bovine and cat based on 100% homology to the immunogen sequence. This sequence shows significant homology to homologues from Xenopus and chicken sources. Reactivity against homologues from other sources is not known.

Species Reactivity :

human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

goat anti-Bmi-1 antibody, Bmi1, B lymphoma Mo MLV insertion region antibody, Bmi 1 antibody, MGC12685 antibody, Murine leukemia viral (bmi 1) oncogene homolog antibody, Oncogene BMI 1 antibody, PCGF 4 antibody

Target Species :

Human

Background :

The Bmi-1 oncogene (also known as polycomb group ring finger 4, MGC12685, murine leukemia viral (bmi 1) oncogene homolog, oncogene BMI 1, polycomb complex protein BMI 1 and RNF51) induces telomerase activity and immortalizes human mammary epithelial cells. Bmi-1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. The polycomb group (PcG) genes are involved in the maintenance of cellular memory through epigenetic chromatin modifications. Recent studies have implicated a role for PcG genes in the self-renewal of hematopoietic stem cells (HSCs), a process in which cellular memory is maintained through cell division. Among the PcG genes, Bmi-1 plays a central role in the inheritance of stemness, and its forced expression promotes HSC self-renewal. These findings highlight the importance of epigenetic regulation in HSC self-renewal and identify PcG genes as potential targets for therapeutic HSC manipulation.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,IF Microscopy,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P35226

NCBI :

27883842

Primary Image Name :

Anti-Bmi1 Antibody - Immunofluorescence Microscopy

more info or order :

Rockland Immunochemicals product webpage