p47ING3 Antibody | Rockland Immunochemicals 600-101-283 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

p47ING3 Antibody

catalog :

600-101-283

quantity :

100 µg

price :

369.00 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-101-283

Name :

Anti-p47 ING3 (GOAT) Antibody - 600-101-283

Display Name :

p47ING3 Antibody

Application Note :

This affinity purified antibody has been tested for use in ELISA against the immunizing peptide. Specific conditions for western blotting reactivity should be optimized by the end user. Expect a band at approximately 47 kDa in size corresponding to ING3 isoform 1 by western blotting in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.10 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000 - 1:40,000

Western Blot Dilution :

1:200 - 1:2,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

ING3

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This affinity purified antibody was prepared from whole Goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 285-310 of Human ING3 protein (Inhibitor of growth family, member 3).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This is an affinity-purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human p47 ING3 protein. This sequence only shows homology to isoform 1 for ING3. The sequence is not present in isoforms 2 and 3. However, 100% homology is on record for this protein from human, dog, rat, chimpanzee and orangutan. Cross reactivity with p47 ING3 protein from mouse and chicken sources is also expected based on high homology (one amino acid change in sequence) by BLAST. Reactivity with p47 ING3 proteins from other sources is not known.

Species Reactivity :

human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

goat anti-p47 ING3 antibody, p47ING3, ING-3, ING 3, Inhibitor of growth family member 3 antibody, Inhibitor of growth protein 3 antibody

Target Species :

Human

Background :

p47 ING3 is a tumor suppressor protein similar to ING1 that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Over-expression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Multiple alternatively spliced transcript variants have been observed. The accession number listed below is for variant (1) that encodes the longest isoform.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2020 Price :

369.00 USD

UniProt :

Q9NXR8

NCBI :

38201655

Primary Image Name :

Structural features of the inhibitor of growth (ING) protein family members.

more info or order :

Rockland Immunochemicals product webpage