IL-35 EBI3 Antibody
Rockland Immunochemicals
catalog: 210-301-B66

mouse monoclonal (V1.4C4.22)
reactivity: mouse
application: western blot, ELISA, immunoprecipitation
citations: 1

ELISA - EBI-3 antibody. Supernatant from 293T cells transfected with a vector containing murine IL-35 was added to an ELISA plate coated with V1.4H6.29 at 2ug/ml in PBS. mEBI3 was detected with biotinylated V1.4C4.22
quantity: 100 µg
price:
to the supplier

IL-35 EBI3 Antibody
Rockland Immunochemicals
catalog: 210-301-C55

mouse monoclonal (V1.4H6.29)
reactivity: mouse
application: western blot, ELISA, immunoprecipitation

Western blot of mouse Anti-Mouse EBI-3 Antibody. Supernatants from cultures containing indicated cells were immunoprecipitated with an anti-p35 mAb plus protein G beads, resolved by SDS page gel and blotted with biotinylated anti mouse EBI-3 clone V1.4H6.29 at 1 µg/ml. The negative and positive controls were supernatants from 293T cells transfected with either vector only (negative) or vector containing murine IL-35 (positive).
quantity: 100 µg
price:
to the supplier

IL-35 EBI3 Antibody
Rockland Immunochemicals
catalog: 210-301-C56

mouse monoclonal (V1.4F5.25)
reactivity: mouse
application: western blot, ELISA, immunoprecipitation

Neutralization: Human CD4+CD25- (Tconv) were purified by FACS from cord blood, activated with anti-CD3/CD28–coated beads and 10IU IL-2 in the presence of hIL-35 and a titration of an isotype control or 210-301-C56 antibody. Percent neutralization was determined by calculating the percent decrease in counts per minute of activated Tconv plus (0%) or minus (100%) human IL-35 in the presence and absence of Ab.
quantity: 100 µg
price:
to the supplier

IL-35 EBI3 Biotin Conjugated Antibody
Rockland Immunochemicals
catalog: 210-306-B66

mouse monoclonal (V1.4C4.22)
reactivity: mouse
conjugate: biotin
application: western blot, ELISA, immunoprecipitation

ELISA - EBI-3 Antibody. Supernatant from 293T cells transfected with a vector containing murine IL-35 was added to an ELISA plate coated with V1.4H6.29 at 2ug/mL in PBS. Mouse EBI-3 was detected with biotinylated V1.4C4.22.
quantity: 100 µg
price:
to the supplier

IL-35 EBI3 Biotin Conjugated Antibody
Rockland Immunochemicals
catalog: 210-306-C55

mouse monoclonal (V1.4H6.29)
reactivity: mouse
conjugate: biotin
application: western blot, ELISA, immunoprecipitation

ELISA assay of EBI-3 Antibody. Supernatant from 293T cells transfected with a vector containing murine IL-35 was added to an ELISA plate coated with V1.4H6.29 (210-301-C55) at 2ug/mL in PBS. Mouse EBI-3 was detected with biotinylated V1.4C4.22 (210-301-B66).
quantity: 100 µg
price:
to the supplier

IL-35 EBI3 Biotin Conjugated Antibody
Rockland Immunochemicals
catalog: 210-306-C56

mouse monoclonal (V1.4F5.25)
reactivity: mouse
conjugate: biotin
application: western blot, ELISA, immunoprecipitation

Neutralization: Human CD4+CD25- (Tconv) were purified by FACS from cord blood, activated with anti-CD3/CD28–coated beads and 10IU IL-2 in the presence of human IL-35 and a titration of an isotype control or 210-301-C56 antibody. Percent neutralization was determined by calculating the percent decrease in counts per minute of activated Tconv plus (0%) or minus (100%) human IL-35 in the presence and absence of Ab.
quantity: 100 µg
price:
to the supplier

IL-35 EBI3 Antibody
Rockland Immunochemicals
catalog: 210-401-B66

domestic rabbit polyclonal
reactivity: mouse, rat
application: western blot, ELISA

Recombinant mouse EBI-3 was loaded on to an SDS-PAGE gel at 0.1 µg and after separation, transferred to nitrocellulose. The expected band is a non-glycosylated polypeptide chain consisting of 207 amino acids and is approximately 23 kDa in size. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
quantity: 100 µg
price:
to the supplier

IL-35 EBI3 Antibody Peroxidase Conjugated
Rockland Immunochemicals
catalog: 210-403-B66

domestic rabbit polyclonal
reactivity: mouse, rat
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

The expected band is a non-glycosylated polypeptide chain consisting of 207 amino acids and is approximately 23 kDa in size corresponding to the mature mouse EBI3 protein by western blotting in appropriate cell lysate or extract.
quantity: 100 µg
price:
to the supplier

IL-35 EBI3 Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 210-406-B66

domestic rabbit polyclonal
reactivity: mouse, rat
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

The expected band is a non-glycosylated polypeptide chain consisting of 207 amino acids and is approximately 23 kDa in size corresponding to the mature mouse EBI3 protein by western blotting in appropriate cell lysate or extract.
quantity: 100 µg
price:
to the supplier

IL-35 EBI3 Antibody
Rockland Immunochemicals
catalog: 210-501-B66

rat monoclonal (10J811)
reactivity: mouse
application: western blot, ELISA, flow cytometry

Flow Cytometry of Anti-Mouse EBI3 Antibody: Figure A: Intracellular flow cytometry of mouse EBI3 in transfected and untransfected HEK 293 cells (Brefeldin A treated, 5 hours) using mouse EBI3 antibody at 3 ug/10^6 cells. Figure B: Intracellular flow cytometry of mouse EBI3 in stimulated and unstimulated RAW cells (LPS treated, 50 ng/ml, overnight) using mouse EBI3 antibody at 1.5 ug/10^6 cells.
quantity: 100 µg
price:
to the supplier

IL-35 EBI3 Biotin Conjugated Antibody
Rockland Immunochemicals
catalog: 210-506-B66

rat monoclonal (10J811)
reactivity: mouse
conjugate: biotin
application: western blot, ELISA, flow cytometry

Western Blot of Mouse Anti-EBI-3 Monoclonal Antibody A) 0.1 ug/mL on recombinant protein B) 3 ug/ml on recombinant mouse IL-35 protein Goat anti-rat IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
quantity: 100 µg
price:
to the supplier

EBI3 Antibody
Rockland Immunochemicals
catalog: 600-401-AY5

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry

quantity: 100 µg
price:
to the supplier

EBI3 Antibody
Rockland Immunochemicals
catalog: 600-401-AY6

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry

quantity: 100 µg
price:
to the supplier