KDEL Receptor Antibody | Rockland Immunochemicals 500-301-F86 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

KDEL Receptor Antibody

catalog :

500-301-F86

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

KR-10

reactivity :

human, mouse, rat, dogs, bovine

application :

western blot

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

500-301-F86

Name :

Anti-KDEL Receptor (MOUSE) Monoclonal Antibody - 500-301-F86

Display Name :

KDEL Receptor Antibody

Application Note :

Anti-KDEL Antibody is suitable for WB, IP, IF microscopy and IHC. Expect a band approximately ~25kDa protein corresponding to the molecular mass of KDEL on SDS-PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

KR-10

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

ASCITES

Gene Name :

KDELR1

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

KDEL Receptor Antibody was produced in mice by repeated immunizations raised against a 21 residue synthetic peptide based on the bovine KDEL receptor and the synthetic peptide coupled to KLH.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.09% (w/v) Sodium Azide

Purity and Specificity :

Anti-KDEL Antibody is clarified mouse ascited fluid. A BLAST analysis was used to suggest cross-reactivity with KDEL from Human, Monkey, Rat, Mouse, Hamster, Rabbit, Pig, Bovine, Sheep, Canine, Chicken, Drosophilia, and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with KDEL from other sources has not been determined. Chaperones, Organelle Markers research.

Species Reactivity :

Human, Monkey, Rat, Mouse, Hamster, Rabbit, Pig, Bovine, Sheep, Canine, Chicken, Drosophilia, Xenopus

Stabilizer :

50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

mouse anti-KDEL Receptor Antibody, ERD2, ERD2.1, ERD21, HDEL, KDEL, KDEL R1, KDELR1, PM23, Lys Asp Glu Leu, K-D-E-L, ER lumen protein retaining receptor 1, KDEL endoplasmic reticulum protein retention, Kdelr1

Target Species :

Bovine

Background :

The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention. There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity; and all are located within the cis-Golgi and its intermediate compartments. In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding. It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P33946

NCBI :

NP_001069963.1

Primary Image Name :

KDEL Immunofluorescence

more info or order :

Rockland Immunochemicals product webpage