MTBP Antibody | Rockland Immunochemicals 500-301-875 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

MTBP Antibody

catalog :

500-301-875

quantity :

100 µL

price :

369.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PHL-1

reactivity :

human

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

500-301-875

Name :

Anti-MTBP (Ascites) (MOUSE) Monoclonal Antibody - 500-301-875

Display Name :

MTBP Antibody

Application Note :

This antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 110 kDa in size corresponding to MTBP by western blotting in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

PHL-1

Concentration Value :

Neat

Conjugation :

(None)

Size :

100 µL

Default Unit :

ELISA Dilution :

1:2,000 - 1:20,000

Western Blot Dilution :

1:200 - 1:2,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

ASCITES

Gene Name :

MTBP

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant amino-terminal HIS 6X -tagged polypeptide corresponding to amino acid residues 667-812 of human MTBP protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This antibody is directed against human MTBP protein. The product was produced from clarified mouse ascetic fluid. A BLAST analysis was used to suggest reactivity with this protein from human (100% homology) and chimpanzee. Cross reactivity with MTBP protein from mouse (77% homology) and chicken (79% homology) is unlikely due to the relatively low number of positive residues and high number of gaps within this sequence. Cross reactivity with MTBP homologues from other sources has not been determined.

Species Reactivity :

human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1 kappa

Synonyms :

mouse anti-MTBP antibody, mouse anti-mouse double minute 2 antibody, MDM 2 BP antibody, MDM2 (mouse double minute 2) binding protein 104kD antibody, MDM2 (mouse double minute 2) binding protein antibody, MDM2 Binding Protein antibody, MDM2 BP antibody

Target Species :

Human

Background :

The MDM2 onco-protein is over expressed in human cancer cells and it promotes tumorigenesis by directly blocking the function of the p53 tumor suppressor. Studies also indicate that MDM2 regulates cell prolifer-ation via p53 independent mechanisms. MTBP (Mdm2 [Two] Binding Protein) was identified by a yeast two-hybrid screen as an MDM2 binding protein (1). MTBP has been reported to modulate cell cycle progression by a p53 independent mechanism and suppress MDM2-mediated p53 degradation when ectopically expressed in cells (1,2). The human MTBP gene localizes to 8q24 (3) and MTBP RNA is expressed in a wide range of tissues, with highest being in the thymus, testes and ovaries (1).

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2020 Price :

369.00 USD

UniProt :

Q96DY7

NCBI :

NP_071328.2

Primary Image Name :

Anti-MTBP Antibody - Western Blot

more info or order :

Rockland Immunochemicals product webpage