product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Phosphotyrosine Antibody
catalog :
500-301-262
quantity :
100 µL
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
13F9
application :
western blot, ELISA
more info or order :
product information
Catalog Number :
500-301-262
Name :
Anti-PhosphoTyrosine (Ascites) (MOUSE) Monoclonal Antibody - 500-301-262
Display Name :
Phosphotyrosine Antibody
Application Note :
This monoclonal antibody has been tested in ELISA and Western Blot and reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and competitive ELISA with phosphoserine or phosphothreonine. The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry, immunohistochemistry and immunoprecipitation. Phosphorylation of tyrosine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.
Clonality :
Monoclonal
Clone ID :
13F9
Conjugation :
(None)
Size :
100 µL
Default Unit :
ul
ELISA Dilution :
1:4,000 - 1:20,000
Western Blot Dilution :
1:500 - 1:2,000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
ASCITES
Host Animal :
Mouse
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
This monoclonal antibody was produced after repeated immunizations of balb/c mice with phosphotyrosine conjugated KLH.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
This product is clarified ascites produced in balb/c mice using clone 13F9. Reactivity is specific for phosphotyrosine and minimal cross reactivity is observed against phosphoserine or phosphothreonine.
Species Reactivity :
phosphotyrosine
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Subclass :
IgG1 kappa
Synonyms :
mouse anti-Phosphotyrosine Antibody, mouse anti-pY antibody, PhosphoTyrosine reactive antibody, monoclonal phosphotyrosine, anti-phosphotyrosine produced in mouse
Background :
Tyrosine (Tyr or Y) or 4-hydroxyphenylalanine is a hydrophilic amino acid. In plants and most microorganisms, tyrosine and α-ketoglutarate are produced via prephenate, when oxidatively decarboxylated with retention of the hydroxyl group to give p-hydroxyphenylpyruvate, which is transaminated using glutamate as the nitrogen source. Mammals synthesize tyrosine from the essential amino acid phenylalanine (phe), which is derived from food. The conversion of phe to tyr is catalyzed by the enzyme phenylalanine hydroxylase causing the addition of a hydroxyl group. In its phosphorylated form, tyrosine is called phosphotyrosine. Tyrosine phosphorylation is one of the key steps in signal transduction and regulation of enzymatic activity. Protein kinases can tag on the hydroxyl group end with a phosphate group to some tyrosine residues causing a phosphorylated form. Tyrosine residues may also be modified by the addition of a sulfate group, a process known as tyrosine sulfation.
Immunogen Type :
Other
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,Western Blot,
Other :
User Optimized
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
Primary Image Name :
Anti-Phosphotyrosine Anitbody - ELISA
more info or order :
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.