SMAD3 phospho S423/phospho S425 Antibody
Rockland Immunochemicals
catalog: 600-401-919

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
citations: 31

Western blot using Rockland's affinity purified anti-Smad3 pS423 pS425 antibody shows detection of endogenous Smad3 in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF (+lanes) for 1 hour. This reagent recognizes phosphorylated Smad3 and has negligible reactivity against non-phosphorylated Smad3 protein. Personal Communication. Ying Zhang, NIH, CCR, Bethesda, MD.

Western blot using Rockland's affinity purified anti-Smad3 pS423 pS425 antibody shows detection of endogenous Smad3 in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF (+lanes) for 1 hour. This reagent recognizes phosphorylated Smad3 and has negligible reactivity against non-phosphorylated Smad3 protein. Personal Communication. Ying Zhang, NIH, CCR, Bethesda, MD.
quantity: 100
price: 499.00 USD
to the supplier

SMAD3 phospho T179 Antibody
Rockland Immunochemicals
catalog: 600-401-C48

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA
citations: 3

The SMAD pathway follows the canonical TGF-ß signaling pathway. TGF-ß dimers bind to a receptor thereby activating the pathway. The type I receptor then recruits and phosphorylates a receptor regulated SMAD (R-SMAD).i.e. SMAD2 or SMAD3. The R-SMAD then binds to the common SMAD (coSMAD) i.e. SMAD4, and forms a heterodimeric complex. This complex then enters the cell nucleus and acts as a transcription factor.
quantity: 100 µg
price:
to the supplier

SMAD3 Antibody
Rockland Immunochemicals
catalog: 600-401-973

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA

Western blot using Rockland's affinity purified anti-Smad3 to detect over-expressed Smad3 in 231 cells (lane 2). Lane 1 shows mock infection of 231 cells with lentiviral vector alone. The membrane was probed with the primary antibody at a 1:5,000 dilution. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD. Personal Communication
quantity: 100 µg
price:
to the supplier

SMAD3 Antibody
Rockland Immunochemicals
catalog: 600-401-920

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry

Western blot using Rockland's affinity purified anti-Smad3 antibody shows detection of endogenous Smad3 in both unstimulated and stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF-ß lanes for 1 hour. This reagent recognizes both non-phosphorylated and phosphorylated Smad3 protein. Personal Communication. Ying Zhang, NIH, CCR, Bethesda, MD.
quantity: 100 µg
price:
to the supplier

SMAD3 Antibody
Rockland Immunochemicals
catalog: 600-401-E70

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western Blot of Rabbit Anti-SMAD3 antibody. Lane 1: AML12 unstimulated. Lane 2: AML12 stimulated with TGFB. Lane 3: MEFwt unstimulated. Lane 4: MEFwt stimulated with TGFB. Lane 5: MEF Smad3 KO unstimulated. Lane 6: MEF Smad3 KO stimulated with TGFB. Lane 7: HEK293 Smad3T179A mutant unstimulated. Lane 8: HEK293 Smad3T179A mutant stimulated with TGFB. Lane 9: HEK293 Smad3T179V mutant unstimulated. Lane 10: HEK293 Smad3T179V mutant stimulated with TGFB. Load: 35 µg per lane. Primary antibody: SMAD 3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 48.1kDa. Other band(s): Smad2pT220.
quantity: 100 µg
price:
to the supplier

Ready-To-Use SMAD3 Antibody
Rockland Immunochemicals
catalog: 600-401-E70-RTU

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation

Ready-To-Use Rabbit Anti-SMAD3 antibody follows the canonical TGF-Beta signaling pathway. TGF-Beta dimers bind to a receptor thereby activating the pathway. The type I receptor then recruits and phosphorylates a receptor regulated SMAD (R-SMAD) .i.e. SMAD2 or SMAD3. The R-SMAD then binds to the common SMAD (coSMAD) i.e. SMAD4, and forms a heterodimeric complex. This complex then enters the cell nucleus and acts as a transcription factor.
quantity: 100 µL
price:
to the supplier