Recombinant scFv Anti-Human CD45 Antibody | Rockland Immunochemicals 400-301-MR2 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Recombinant scFv Anti-Human CD45 Antibody

catalog :

400-301-MR2

quantity :

100 µg

clonality :

recombinant

host :

mouse

conjugate :

nonconjugated

clone name :

BC8

reactivity :

human

application :

ELISA, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Immunofluorescence of scFv fragment Anti-CD45 Antibody. Tissue: Human Tonsil FFPE. Antibody: Recombinant scFv fragment Anti-Human CD45 (Mouse) Antibody used at 0.8mg/mL. Permeabilization: 0.1% triton. Counterstain: DAPI. Predicted localization/observed: Shows specific cellular binding (mostly in the germinal centers of lymphoid follicles and some surrounding area such a stratified squamous epithelium). Results: The reagent worked well at ready to use concentration only and showed regional specificity.

product information

Catalog Number :

400-301-MR2

Name :

Recombinant scFv fragment Anti-Human CD45 (Mouse) Antibody - 400-301-MR2

Display Name :

Recombinant scFv Anti-Human CD45 Antibody

Application Note :

Recombinant scFv fragment Anti-Human CD45 Monoclonal Antibody has been tested in ELISA Capture, Flow Cytometry, Immunofluorescence. This antibody has been diluted for recommended use per test. Typically, we use 1×10^6 cells in a 100μL experimental sample (a test). Optimal titers for applications should be determined by the researcher.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Recombinant Monoclonal

Clone ID :

BC8

Concentration Value :

0.71 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

User Optimized

Flow Cytometry Dilution :

User Optimized

IF Microscopy Dilution :

User Optimized

Expiration :

Expiration date is eighteen (18) months from date of opening.

Format :

IgG1

Gene Name :

PTPRC

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Human Peripheral Blood Leukocytes

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.09% (w/v) Sodium Azide

Purity and Specificity :

The scFv antibody fragment was purified from disrupted Escherichia coli culture by column chromatography.

Species Reactivity :

Human Leukocytes

Storage :

Store undiluted at -20° C prior to opening. Protect from prolonged exposure to light.

Subclass :

IgG1

Synonyms :

PTPRD; CD45R; LCA; L-CA; Leukocyte Common Antigen

Target Species :

Human

Background :

CD45 is encoded by the PTPRC (Protein tyrosine phosphatase receptor type C) gene. CD45, also known as the leukocyte common antigen (LCA), is a member of the protein tyrosine phosphatase (PTP) family. It is present on all human leukocytes including lymphocytes, monocytes, granulocytes, eosinophils, and thymocytes. CD45 is absent from circulating erythrocytes, platelets, or mature erythroid cells of bone marrow and non-hemopoietic tissues. This is an anti-cd45 scFv antibody fragment. This scFv fragment representing the functional antigen binding fragment of immunoglobulins is produced in bacterial expression systems. Using biochemical procedures and/or genetic modification (recombinant DNA technology), these fragments can be coupled to compounds and proteins that allow for the detection of these fragments. Examples of such compounds include fluorophores (e.g. fluorescein) and enzymes (e.g. alkaline phosphatase) generating fluorescence. The resultant compounds now have bifunctional properties in that they can bind to their target via the scFv fragment and can be detected via their fluorescence marker. In addition to labeling the scFv with fluorophore markers, the scFv antibody fragment has been coupled to magnetic microparticles allowing for the capture of the scFv target complex in a magnetic field. An scFv fragment retains the complete antigen-binding capability of the whole monoclonal antibody, thus facilitating a unique molecule with the property of being used as either a diagnostic or therapeutic reagent. In addition, the scFv with exquisite specificity and affinity towards a specific target can also be used as a delivery vehicle for radioimmunoimaging and radioimmunotherapy. In addition, the small antigen-binding scFv offer several advantages over a whole antibody molecule in that the smaller size of the scFv allow it to penetrate more rapidly and evenly to tumors and can penetrate the blood brain-barrier. scFv fragments have more rapid clearance from blood, and therefore be coupled with drugs and radionuclides in order to result in low exposure of the healthy tissue. Also, there appears to be no uptake of the scFv by the kidney allowing them to efficiently localize to the tumors, an important property in cancer therapy.

Immunogen Type :

Native Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Flow Cytometry,IF Microscopy,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P08575

NCBI :

NP_002829.3

Primary Image Name :

Immunofluorescence of scFv fragment Anti-CD45 Antibody

New Product :

Yes

more info or order :

Rockland Immunochemicals product webpage