Recombinant Anti-TNF alpha Fab Antibody | Rockland Immunochemicals 400-001-MT3 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Recombinant Anti-TNF alpha Fab Antibody

catalog :

400-001-MT3

quantity :

price :

465.00 USD

clonality :

recombinant

conjugate :

nonconjugated

clone name :

Abz520

reactivity :

human

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Western Blot using Adalimumab, Anti-human TNF alpha Fab fragment to detect human TNA alpha. Lanes 1, 3, and 5: TNF alpha reduced. Lanes 2, 4, 6: TNF alpha non-reduced. MW: Molecular Weight Ladder. Protein Load: Lane 1-2: 200ng. Lane 3-4: 40ng. Lane 5-6: 8ng. Primary Antibody: Anti-TNF alpha Fab fragment Antibody at 1.0µg/mL for 2 hours at 4°C. Secondary Antibody: F(ab')2 Human IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed (p/n 709-1318) at 1:5,000 dilution was used. The protein has a calculated MW of 18.3kDa. The protein migrates as 18.2kDa under reducing (R) and non-reducing condition (SDS-PAGE).

product information

Catalog Number :

400-001-MT3

Name :

Humanized Recombinant Anti-Human TNFα Fab fragment Antibody - 400-001-MT3

Display Name :

Recombinant Anti-TNF alpha Fab Antibody

Application Note :

Humanized Recombinant Anti-Anti-TNFα Fab fragment Antibody has been tested for use in Western Blot and ELISA. This antibody recognizes structured TNFα and will work in western blot when the protein has not been denatured with DTT or bMe. Although not tested, this antibody could be useful in in IHC and in in-vivo and other cellular assays. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Recombinant Monoclonal

Clone ID :

Abz520

Concentration Value :

0.42 mg/ml

Conjugation :

(None)

Size :

Default Unit :

µg

ELISA Dilution :

User Optimized

Western Blot Dilution :

User Optimized

Expiration :

Expiration date is six (6) months from date of opening.

Format :

IgG Fab

Gene Name :

TNF alpha

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Purity and Specificity :

Humanized Recombinant TNFα Fab fragment was expressed in CHO cells. The purity was estimated to be >95% by SDS-PAGE analysis.

Species Reactivity :

H. sapiens (Human)

Tested Applications :

ELISA; WB

Stabilizer :

30% Glycerol

Storage :

Store vial at -20° C. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody, Tumour Necrosis Factor Alpha antibody, rabbit anti-Tumor Necrosis Factor Alpha Antibody, rabbit anti-TNF Alpha Antibody, Tumor necrosis factor, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2

Target Species :

Human

Background :

Anti-TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-α) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNF is an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD). Anti-TNF alpha antibody, Adalimumab, a monoclonal antibody, working by inactivating TNFα is approved to treat rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, Crohn's disease, ulcerative colitis, psoriasis, hidradenitis suppurativa, uveitis, and juvenile idiopathic arthritis and sold under the brand name Humira. This antibody is made from a Adalimumab Fab fragment making it specific, efficient, and effective. Humanized Recombinant Anti-TNFα Fab fragment Antibody is useful for researchers interested in Cancer and Inflammatory disease research.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2023 Price :

465.00 USD

UniProt :

P01375

NCBI :

7124

Primary Image Name :

Western Blot of Anti-human TNF alpha Fab fragment Antibody

more info or order :

Rockland Immunochemicals product webpage