Recombinant Anti-CTLA4 Fab Antibody | Rockland Immunochemicals 400-001-MT1 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Recombinant Anti-CTLA4 Fab Antibody

catalog :

400-001-MT1

quantity :

price :

465.00 USD

clonality :

recombinant

conjugate :

nonconjugated

clone name :

Abz521

reactivity :

human

application :

western blot, ELISA, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Western Blot using Humanized Recombinant Ipilimumab (Yervoy) Anti-human CTLA4 Fab fragment Antibody to detect extracellular domain of CTLA4 protein. MW: Molecular Weight Ladder. Lanes 1, 3, and 5: CTLA-4 reduced. Lanes 2, 4, 6: CTLA-4 non-reduced. Protein Load: Lane 1-2: 200ng. Lane 3-4: 40ng. Lane 5-6: 8ng. Primary Antibody: Anti-CTLA4 Fab fragment Antibody at 1.0µg/mL for 2 hours at 4°C. Secondary Antibody: F(ab')2 Human IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed (p/n 709-1318) at 1:5,000 dilution. The protein has a calculated MW of 18.8kDa. The protein migrates ~23-30kDa under reducing (R) conditions and ~45-60kDa in a native form in SDS-PAGE due to glycosylation.

product information

Catalog Number :

400-001-MT1

Name :

Humanized Recombinant Anti-Human CTLA4 Fab fragment Antibody - 400-001-MT1

Display Name :

Recombinant Anti-CTLA4 Fab Antibody

Application Note :

Humanized Recombinant Anti-CTLA-4 Fab fragment Antibody is tested for use in Flow Cytometry, Western Blot, and ELISA. This antibody recognizes structured CTLA-4 and will work in western blot when the protein has not been denatured with DTT or bMe. Although not tested, this antibody could be useful in in IHC and in in-vivo and other cellular assays. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Recombinant Monoclonal

Clone ID :

Abz521

Conjugation :

(None)

Size :

Default Unit :

µg

ELISA Dilution :

User Optimized

Flow Cytometry Dilution :

User Optimized

Western Blot Dilution :

User Optimized

Expiration :

Expiration date is six (6) months from date of opening.

Format :

IgG Fab

Gene Name :

CTLA4

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Purity and Specificity :

Humanized Recombinant CLTA4 Fab fragment was expressed in CHO cells. The purity was estimated to be >95% by SDS-PAGE analysis.

Species Reactivity :

H. sapiens (Human)

Tested Applications :

ELISA; FC; SDS-PAGE; WB

Stabilizer :

30% Glycerol

Storage :

Store vial at 2-8° C. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

Cytotoxic T-Lymphocyte Associated Protein 4, Ipilimumab, Yervoy, recombinant Monoclonal CTLA4, RabMAb CTLA4, CTLA-4, CD152, Insulin-Dependent Diabetes Mellitus 12, Cytotoxic T-Lymphocyte Protein 4, Celiac Disease 3, GSE, CD, Ligand And Transmembrane Spliced, Cytotoxic T Lymphocyte Associated Antigen 4, Cytotoxic T Lymphocyte Associated Antigen 4 Short Spliced Form, Cytotoxic T-Lymphocyte-Associated Serine Esterase-4, Cytotoxic T-Lymphocyte-Associated Antigen 4, CD152 Isoform, CD152 Antigen, CELIAC3, IDDM12, ALPS5, GRD4

Target Species :

Human

Background :

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that functions as an immune checkpoint and downregulates immune responses. CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells. CTLA4 is a member of the immunoglobulin superfamily. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. T cell activation through the T cell receptor and CD28 leads to increased expression of CTLA-4, an inhibitory receptor for B7 molecules. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis. This antibody is made from a humanized Fab fragment of anti-CTLA4, ipilimumab or Yervoy, the first FDA approved immune checkpoint blockade therapy for cancer treatment. Humanized Recombinant Anti-CTLA4 fragment Antibody is useful for researchers interested in cancer research, immunodeficiency, and autoimmune diseases.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Flow Cytometry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2023 Price :

465.00 USD

UniProt :

P16410

NCBI :

1493

Primary Image Name :

Western Blot Results of Humanized Recombinant Anti-human CTLA4 Fab fragment Antibody

more info or order :

Rockland Immunochemicals product webpage