Recombinant DIG (DIG45) Antibody | Rockland Immunochemicals 400-001-MG2 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Recombinant DIG (DIG45) Antibody

catalog :

400-001-MG2

quantity :

50 µg

clonality :

recombinant

host :

llama

conjugate :

nonconjugated

clone name :

DIG45

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

SDS-PAGE Results of Recombinant Anti-DIG Single Domain Antibody. Lane 1: Anti-DIG-VhH (Clone DIG45) Reduced (1µg). Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: Anti-DIG-VhH (Clone DIG45) Non-Reduced (1µg). 4-20% Gel Coomassie Blue stained. Expected MW: ~15kDa. Observed MW: ~16kDa.

product information

Catalog Number :

400-001-MG2

Name :

Anti-DIG (VHH) Single Domain Antibody - 400-001-MG2

Display Name :

Recombinant DIG (DIG45) Antibody

Application Note :

Anti-DIG is a his-tagged monoclonal recombinant antibody designed to detect digoxigenin and its conjugates. This antibody is intended for use in immunological assays including ELISA, western blotting, immunofluorescence and fluorescence activated cell sorting (FACS). Fluorochrome conjugated anti-DIG can be used for detection of sensitive non-radioactive in situ hybridization probes. The antibody can be labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection of DIG-conjugated molecules by immunoblotting or immunofluorescence. Secondary detection can be achieved using conjugated anti-His tag or anti-VHH antibodies. Optimal titers for applications should be confirmed by the end user.

Buffer :

1X PBS, pH 7.4

Clonality :

Recombinant Monoclonal

Clone ID :

DIG45

Concentration Value :

1.0 mg/mL

Concentration Definition :

by modified Lowry assay

Conjugation :

(None)

Size :

50 µg

Default Unit :

µg

ELISA Dilution :

1:2000

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Gene Name :

Digoxigenin

Host Animal :

Llama

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-DIG (VHH) Antibody is a recombinant antibody. The clone was isolated from a library prepared from a hyper-immunized llama host and purified by affinity chromatography from bacterial culture. Expect reactivity against DIG-labeled probes and proteins.

Species of Origin :

E. coli

Species Reactivity :

Digitalis sp.

Stabilizer :

20% (v/v) Glycerol

Storage :

Store at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

anti-Digoxigenin, Digoxigenin (DIG), 1672-46-4, Lanadigenin, Digoxigenine, UNII-NQ1SX9LNAU, DIG, CHEBI:42098, HSDB 7108, NQ1SX9LNAU, EINECS 216-806-2, BRN 0096479, 4-(3,12,14-Trihydroxy-10,13-Dimethyl-Hexadecahydro-Cyclopenta[a]Phenanthren-17-Yl)-5h-Furan-2-One

Background :

Digoxigenin is a cardenolide steroid isolated from the plant species of the genus Digitalis. It has a role as a hapten and a plant metabolite and can be obtained by hydrolysis of digoxin. Digoxigenin or DIG is one of the most common reporters used with nucleotides and requires the use of a conjugated anti-digoxigenin antibody. Since digoxigenin is a plant-related chemical not found in mammalian tissues, it is widely used as a method for generating non-radioactive probes, and is an alternative to biotin. DIG is an all-purpose immuno-tag, and in particular a standard marker for immunohistochemistry and in situ hybridization that supports both chromogenic and chemiluminescence detection. A single-domain antibody (sdAb) is a small antibody fragment consisting of the monomeric variable domain derived from camelid heavy chain-only immunoglobulins naturally found in llamas, alpacas and camels. Also known as VHH antibodies, these are the smallest functional antigen-binding fragment that occurs in nature (12 - 14 kDa) and are now being used in biotechnology as a novel antibody scaffold. The small size of the VHH single domain antibody makes it very attractive for use in diagnostic imaging and potentially therapeutic applications.

Epitope Tag Type :

6X HIS

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

Primary Image Name :

SDS-PAGE of Recombinant Anti-DIG (VHH) Single Domain Antibody

New Product :

Yes

more info or order :

Rockland Immunochemicals product webpage