V5 Epitope Tag Antibody
Rockland Immunochemicals
catalog: 600-401-378
domestic rabbit polyclonal
application: western blot, ELISA, immunohistochemistry
citations: 3

Anti-V5 epitope tag polyclonal antibody detects V5-tagged recombinant protein by western blot. This antibody was used at 1.0 µg/ml to detect 0.05 µg (lane 2) of full-length recombinant mouse serum albumin containing the V5 epitope tag at the carboxy end. Comparison to MW markers (lane 1) indicates detection of monomeric V5 tagged albumin. A 4-20% gradient gel was used to separate the protein by SDS-PAGE under non-reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody overnight at 4° C followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 45 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
quantity: 100 µg
price:
to the supplier
V5 Epitope Tag Antibody Dylight™ 488 Conjugated
Rockland Immunochemicals
catalog: 600-441-378
domestic rabbit polyclonal
conjugate: DyLight488
application: western blot

quantity: 100 µg
price:
to the supplier
V5 Epitope Tag Antibody Dylight™ 549 Conjugated
Rockland Immunochemicals
catalog: 600-442-378
domestic rabbit polyclonal
conjugate: DyLight549
application: western blot

Properties of DyLight™ Fluorescent Dyes.
quantity: 100 µg
price:
to the supplier
V5 Epitope Tag Antibody Dylight™ 649 Conjugated
Rockland Immunochemicals
catalog: 600-443-378
domestic rabbit polyclonal
conjugate: DyLight649
application: western blot


quantity: 100 µg
price:
to the supplier
V5 Epitope Tag Antibody Dylight™ 680 Conjugated
Rockland Immunochemicals
catalog: 600-444-378
domestic rabbit polyclonal
conjugate: DyLight680
application: western blot

DyLight™ dyes can be used for two-color Western Blot detection with low background and high signal.  Anti-tubulin was detected using a DyLight™ 680 conjugate.  Anti-TNFa was detected using a DyLight™ 800 conjugate. The image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
quantity: 100 µg
price:
to the supplier
V5 Epitope Tag Antibody Dylight™ 800 Conjugated
Rockland Immunochemicals
catalog: 600-445-378
domestic rabbit polyclonal
conjugate: DyLight800
application: western blot

quantity: 100 µg
price:
to the supplier
V5 Epitope Tag Antibody Dylight™ 405 Conjugated
Rockland Immunochemicals
catalog: 600-446-378
domestic rabbit polyclonal
conjugate: DyLight 405
application: western blot

quantity: 100 µg
price:
to the supplier