6X His Epitope Tag Antibody
Rockland Immunochemicals
catalog: 600-401-382

domestic rabbit polyclonal
application: western blot, ELISA, immunocytochemistry, dot blot
citations: 9

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. The blot was blocked with 3% BSA in TBST for 45 min at RT. Antibody was incubated with blot at a 1:1,000 dilution in TBST with 3% BSA for 1 hour at RT. Detection occurred using HRP Gt-a-Rabbit IgG (p/n 611-103-127) diluted 1:80,000 in blocking buffer (p/n MB-070) for 30 min at RT. Lane 1 was loaded with 12-Epitope Tag Protein Marker Lysate (p/n MB-301-0100) which has the His epitope tag incorporated through a C-terminal linkage (~42 kDa). Lane 2 was loaded with His-SUMO-GFP recombinant protein which has the His epitope tag incorporated through an N-terminal linkage (~40 kDa). A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. Proteins were transferred to nitrocellulose using standard methods. Molecular weights were estimated by comparison to standards (lane M).
quantity: 100
price: 335.00 USD
to the supplier

6X His Epitope Tag Antibody
Rockland Immunochemicals
catalog: 200-301-382

mouse monoclonal (33D10.D2.G8)
application: western blot, ELISA
citations: 5

Western Blot using Rockland Immunochemicals' Mouse Anti-6x-His Epitope Tag Monoclonal Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). In lane 1 are molecular weight markers.

Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.

Western Blot using Rockland Immunochemicals' Mouse Anti-6x-His Epitope Tag Monoclonal Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). In lane 1 are molecular weight markers.
quantity: 100
price: 359.00 USD
to the supplier

6X His Tag Antibody DyLight™ 649
Rockland Immunochemicals
catalog: 200-343-382

mouse monoclonal (33D10.D2.G8)
conjugate: DyLight649
application: western blot, ELISA, dot blot
citations: 5

DyLight™ dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight™ 549 conjugate. Anti-TNF? was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.
quantity: 100
price: 480.00 USD
to the supplier

6X His Tag Antibody HRP Conjugated
Rockland Immunochemicals
catalog: 200-303-382

mouse monoclonal (33D10.D2.G8)
conjugate: HRP
application: western blot, ELISA
citations: 2

ELISA of Mouse anti-6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE-1000).

Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.
quantity: 100
price: 480.00 USD
to the supplier

6X His Tag Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 200-306-382

mouse monoclonal (33D10.D2.G8)
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
citations: 2

ELISA of Mouse anti-6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE-1000).

Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.
quantity: 100
price: https://www.rockland.com/search/?searchString=200-306-382
to the supplier

6X His Tag Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 600-406-382

domestic rabbit polyclonal
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
citations: 2

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot.  Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 µg/ml was used to detect 1.0 µg of recombinant protein containing the His epitope tag.  A 4-20% gradient gel was used to resolve the protein by SDS-PAGE.  The protein was transferred to nitrocellulose using standard methods.  After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (code 611-132-122) for 30 min at room temperature.  LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.  Other detection systems will yield similar results.
quantity: 100 µg
price:
to the supplier

6X His Epitope Tag Antibody Peroxidase Conjugated
Rockland Immunochemicals
catalog: 600-403-382

domestic rabbit polyclonal
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
citations: 1

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot.  Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 µg/ml was used to detect 1.0 µg of recombinant protein containing the His epitope tag.  A 4-20% gradient gel was used to resolve the protein by SDS-PAGE.  The protein was transferred to nitrocellulose using standard methods.  After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (code 611-132-122) for 30 min at room temperature.  LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.  Other detection systems will yield similar results.
quantity: 100 µg
price:
to the supplier

6X His Tag Antibody Dylight™ 800 Conjugated
Rockland Immunochemicals
catalog: 200-345-382

mouse monoclonal (33D10.D2.G8)
conjugate: DyLight800
application: western blot, ELISA, dot blot
citations: 1

DyLight™ dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight™ 680 conjugate. Anti-TNF? was detected using a DyLight™ 800 conjugate. The image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
quantity: 100
price: 480.00 USD
to the supplier

6X His Tag Antibody
Rockland Immunochemicals
catalog: 200-301-B90

mouse monoclonal (33D10.D2.G8)
application: western blot, ELISA, immunohistochemistry

Western Blot using Rockland Immunochemical's Mouse Anti-6x-His Epitope Tag Monoclonal Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). In lane 1 are molecular weight markers.

Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.

Western Blot using Rockland Immunochemical's Mouse Anti-6x-His Epitope Tag Monoclonal Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). In lane 1 are molecular weight markers.
quantity: 1 mg
price:
to the supplier

6X His Tag Antibody Dylight™ 488 Conjugated
Rockland Immunochemicals
catalog: 200-341-382

mouse monoclonal (33D10.D2.G8)
conjugate: DyLight488
application: western blot

DyLight™ dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. DyLight™ dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods. This image shows anti-histone detection using a DyLight™ 488 conjugate (green). Anti-Tubulin was detected using a DyLight™ 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).

Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.
quantity: 100 µg
price:
to the supplier

6X His Tag Antibody Dylight™ 549 Conjugated
Rockland Immunochemicals
catalog: 200-342-382

mouse monoclonal (33D10.D2.G8)
conjugate: DyLight549
application: western blot

Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.
quantity: 100 µg
price:
to the supplier

6X His Tag Antibody Dylight™ 680 Conjugated
Rockland Immunochemicals
catalog: 200-344-382

mouse monoclonal (33D10.D2.G8)
conjugate: DyLight680
application: western blot

Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.
quantity: 100 µg
price:
to the supplier

6X His Tag Antibody Dylight™ 405 Conjugated
Rockland Immunochemicals
catalog: 200-346-382

mouse monoclonal (33D10.D2.G8)
conjugate: DyLight 405
application: western blot

Properties of DyLight™ Fluorescent Dyes.

Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.
quantity: 100 µg
price:
to the supplier

6X His Tag Antibody Dylight™ 488 Conjugated
Rockland Immunochemicals
catalog: 600-441-382

domestic rabbit polyclonal
conjugate: DyLight488
application: western blot

DyLight™ dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. DyLight™ dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods. This image shows anti-histone detection using a DyLight™ 488 conjugate (green). Anti-Tubulin was detected using a DyLight™ 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).
quantity: 100 µg
price:
to the supplier

6X His Tag Antibody Dylight™ 549 Conjugated
Rockland Immunochemicals
catalog: 600-442-382

domestic rabbit polyclonal
conjugate: DyLight549
application: western blot

DyLight™ dyes can be used for two-color Western Blot detection with low background and high signal.  Anti-tubulin was detected using a DyLight™ 549 conjugate.  Anti-TNFa was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.
quantity: 100 µg
price:
to the supplier

6X His Tag Antibody Dylight™ 649 Conjugated
Rockland Immunochemicals
catalog: 600-443-382

domestic rabbit polyclonal
conjugate: DyLight649
application: western blot

quantity: 100 µg
price:
to the supplier

6X His Tag Antibody Dylight™ 680 Conjugated
Rockland Immunochemicals
catalog: 600-444-382

domestic rabbit polyclonal
conjugate: DyLight680
application: western blot

Properties of DyLight™ Conjugates.
quantity: 100 µg
price:
to the supplier

6X His Tag Antibody Dylight™ 800 Conjugated
Rockland Immunochemicals
catalog: 600-445-382

domestic rabbit polyclonal
conjugate: DyLight800
application: western blot

quantity: 100 µg
price:
to the supplier

6X His Tag Antibody Dylight™ 405 Conjugated
Rockland Immunochemicals
catalog: 600-446-382

domestic rabbit polyclonal
conjugate: DyLight 405
application: western blot

quantity: 100 µg
price:
to the supplier

6X His Tag CY3 Conjugated Antibody
Rockland Immunochemicals
catalog: 200-304-382

mouse monoclonal (33D10.D2.G8)
conjugate: Cy3
application: flow cytometry

quantity: 50 µg
price:
to the supplier

6X His Tag CY5 Conjugated Antibody
Rockland Immunochemicals
catalog: 200-310-382

mouse monoclonal (33D10.D2.G8)
conjugate: Cy5
application: flow cytometry

quantity: 50 µg
price:
to the supplier

6X His Tag ATTO 550 Conjugated Antibody
Rockland Immunochemicals
catalog: 200-354-382

mouse monoclonal (33D10.D2.G8)
application: western blot

quantity: 50 µg
price:
to the supplier

6X His Tag ATTO 647N Conjugated Antibody
Rockland Immunochemicals
catalog: 200-356-382

mouse monoclonal (33D10.D2.G8)
conjugate: ATTO 647N
application: western blot

quantity: 50 µg
price:
to the supplier