domestic rabbit polyclonal
reactivity: human
application: western blot, dot blot
citations: 1
reactivity: human
application: western blot, dot blot
citations: 1
Western blot using Rockland's anti-IL6 antibody. Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band ~21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).
quantity: 1
price: 1,292.00 USD
to the supplier
mouse monoclonal (33A12.G9)
reactivity: human
application: western blot, ELISA, flow cytometry
reactivity: human
application: western blot, ELISA, flow cytometry
Western Blot showing detection of Human IL-6. 100 ng of Human IL-6 was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C, Anti-Human IL-6 (MOUSE) Antibody (p/n 209-301-310) was used at 1:1000 in 1% BSA-TTBS over night at 4°C. Peroxidase conjugated Rabbit Anti-mouse secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) at 1:40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc® 4000 MP. Band indicates correct 17 kDa molecular weight position expected for Human IL-6.
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
reactivity: human
application: western blot, ELISA, immunohistochemistry
Western blot using Rockland's anti-Human IL6 antibody shows detection of a band ~24 kDa in size corresponding to recombinant human IL6 (arrowhead). Molecular weight markers are also shown. After transfer, the membrane was blocked overnight with 1% BSA in TTBS followed by reaction with primary antibody at a 1:500 dilution. Detection occurred using HRP conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) and FemtoMax™ Chemiluminescent substrate (p/n FEMTOMAX-110). Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
Western Blot of Peroxidase conjugated Rabbit anti-Human IL-6 antibody. Lane 1: Human IL-6. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase Human IL-6 secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 20 kDa for Human IL-6. Other band(s): none.
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry