domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, blocking or activating experiments
citations: 2
reactivity: human
application: western blot, ELISA, immunohistochemistry, blocking or activating experiments
citations: 2
IL-1B was used at a 1:200 dilution incubated 1 h at room temperature to detect dog IL-1B by Western blot. Lane 1 and 2 were loaded with 2.5 ?g and 500 ng of dog IL-1B respectively. The molecular weight of the detected band is estimated by comparison to molecular weight markers (not shown). Detection occurred using a 1:3,000 dilution of IRDye™800 conjugated Donkey anti-Rabbit IgG (code # 611-732-127) for 1h at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
quantity: 1
price: 1,292.00 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, dogs
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
reactivity: human, dogs
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
domestic rabbit polyclonal
reactivity: human, dogs
application: western blot, ELISA
reactivity: human, dogs
application: western blot, ELISA
Western blot showing detection of recombinant human IL-1ß by Rockland's anti-IL-1ß antibody. Recombinant human IL-1ß was loaded on to an SDS-PAGE gel at 0.1 µg and after separation, transferred to nitrocellulose. The expected band is approximately 18 kDa in size. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: human, dogs
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
reactivity: human, dogs
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
domestic rabbit polyclonal
reactivity: human, dogs
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
reactivity: human, dogs
conjugate: biotin
application: western blot, ELISA, immunohistochemistry