HSP90 K294 Antibody
Rockland Immunochemicals
catalog: 600-401-981

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
citations: 2

Rockland's affinity purified anti-Hsp90 acetyl K294 antibody was used at 20 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and granular cytoplasmic positive staining in human prostate carcinoma at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.
quantity: 100 µg
price:
to the supplier

HSP90 alpha Antibody
Rockland Immunochemicals
catalog: 600-401-929

domestic rabbit polyclonal
reactivity: human, mouse, rat, chicken
application: western blot, ELISA, immunohistochemistry

Western blot using Rockland's Affinity Purified anti-Hsp90 antibody shows detection of a band at ~90 kDa corresponding to Hsp90 in various lysate preparations (arrowhead). Cos7 transfected with mutant K294R Hsp90 (left two lanes), Cos7 transfected with wild type Hsp90 (middle two lanes) and empty vector Cos7 cells (right two lanes) were either treated with Trichostatin A (an HDAC inhibitor) or mock treated with DMSO only, as indicated. Transfected cells express either mutant or wt Hsp90 coupled to FlagTM tag. Anti-FlagTM immunoprecipitation was performed prior to western blotting with anti-Hsp90. Personal Communication, Brad Scroggins, Urologic Oncology Branch, CCR, NCI, Rockville, MD.
quantity: 100 µg
price:
to the supplier

HSP90 Antibody
Rockland Immunochemicals
catalog: 200-301-F67

mouse monoclonal (AC-16)
reactivity: human, mouse, rat, chicken
application: western blot, immunohistochemistry

Immunohistochemistry of mouse anti-Hsp90 antibody. Tissue: inflammatory cells and epithelia mucosa in mouse colon tissues. Primary Antibody: Hsp90 antibody at 1 µg/mL for 1h at RT. Secondary antibody: Peroxidase mouse secondary at 1:10,000 for 45 min at RT. Localization: Cytoplasm. Staining: Hsp90 as brown signal.
quantity: 200 µg
price:
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Hsp90 Antibody
Rockland Immunochemicals
catalog: 200-301-F68

mouse monoclonal (D7A)
reactivity: human, mouse, rat, chicken, bovine
application: western blot, ELISA, immunohistochemistry, immunoprecipitation

Immunohistochemistry of mouse anti-Hsp90 antibody. Tissue: mouse colon tissue. Primary Antibody: Hsp90 antibody at 1 µg/mL for 1h at RT. Secondary antibody: Peroxidase mouse secondary at 1:10,000 for 45 min at RT. Localization: Cytoplasm. Staining: Hsp90 as brown signal.
quantity: 100 µg
price:
to the supplier

HSP90 alpha Antibody
Rockland Immunochemicals
catalog: 200-301-F69

mouse monoclonal (2G5.G3)
reactivity: human, mouse, rat
application: western blot, ELISA

Immunofluorescence of mouse anti-Hsp90 alpha antibody. Tissue: human keratinocyte line HaCat. Antigen retrieval: not required. Primary Antibody: Hsp90 alpha antibody at 10ug/ml for 1h at RT. Secondary antibody: fluorescein mouse secondary at 1:10,000 for 45 min at RT. Localization: cytoplasmic. Staining: Hsp90 alpha as green fluorescent signal.
quantity: 200 µg
price:
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HSP90 complex Antibody
Rockland Immunochemicals
catalog: 200-301-F73

mouse monoclonal (8D3)
reactivity: human, mouse, rat

SDS-PAGE of Mouse anti-Hsp90 complex antibody. Lane 1: Hsp90 complex IP from rabbit reticulocyte lysate. Lane 2: Hsp90 complex IP from rabbit reticulocyte lysate. Load: 35 µg per lane. Predicted/Observed size: 83.3 kDa, 128 kDa for Hsp90 complex. Other band(s): Hsp90, IgM, Hsp70, Hop, IgG.
quantity: 200 µg
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HSP90 total Antibody
Rockland Immunochemicals
catalog: 200-301-F74

mouse monoclonal (4F3.E8)
reactivity: human, mouse, rat
application: western blot, ELISA

Western Blot of mouse anti-Hsp90 total antibody. Lane 1: rat tissues. Lane 2: none. Load: 35 µg per lane. Primary antibody: Hsp90 total antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 84.7 kDa/~85 for Hsp90 total. Other band(s): none.
quantity: 200 µg
price:
to the supplier

HSP90 alpha/beta Antibody
Rockland Immunochemicals
catalog: 200-301-F75

mouse monoclonal (Hyb-K41220A)
reactivity: human, rat
application: western blot, ELISA

Western Blot of mouse anti-Hsp90 total antibody. Lane 1: A431. Lane 2: A549. Lane 3: HCT116. Lane 4: HeLa. Lane 5: HEK293. Lane 6: HepG2. Lane 7: HL-60. Lane 8: HUVEC. Lane 9: Jurkat. Lane 10: MCF7. Lane 11: PC3. Lane 12: T98G. Lane 13: Rat Brain. Load: 10 µg per lane. Primary antibody: Hsp90 total antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 84.7 kDa/~90for Hsp90 total. Other band(s): none.
quantity: 100 µg
price:
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HSP90 alpha Antibody
Rockland Immunochemicals
catalog: 209-301-F70

mouse monoclonal (Hyb-K41009)
reactivity: human
application: western blot, ELISA, immunohistochemistry

Immunohistochemistry of mouse anti-Hsp90 antibody. Tissue: inflammatory cells in mouse colon tissues. Primary Antibody: Hsp90 antibody at 1 µg/mL for 1h at RT. Secondary antibody: Peroxidase mouse secondary at 1:10,000 for 45 min at RT. Localization: Cytoplasm. Staining: Hsp90 as brown signal.
quantity: 100 µg
price:
to the supplier