domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section, dot blot
citations: 136
reactivity: human, mouse, rat, bovine
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section, dot blot
citations: 136
Western blot analysis is shown using Rockland's Affinity Purified anti-Collagen I antibody to detect expression of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane). Protein staining shown below each blot depicts equal protein loading. An equal amount of the whole cell protein (100 µg) was separated by SDS-PAGE and electroblotted to nitro-cellulose membranes. Proteins were detected by incubating the membrane with anti-Collagen I antibody at a concentration of 0.2–2 µg/10 ml in TBS (100 mM Tris-HCl, 0.15 M NaCl, pH 7.4) with 5% Non-fat milk. Detection occurred by incubation with a horseradish peroxidase-conjugated secondary antibody at 1 µg/10 ml. Proteins were detected by a chemiluminescent method using the PIERCE ECL kit (Amersham Biosciences). Other detection systems will yield similar results. See Hazra et al. (2004) for additional details.
Western blot of Human Collagen Type I. Lane 1: Human Collagen Type 1. Lane 2: None. Load: 50 ng per lane. Primary antibody: Collagen Type I antibody at 1:1,000 overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 139 & 130 kDa, 139 & 130 kDa for Collagen Type I. Other Band(s): Collagen Type I splice variants and isoforms.
quantity: 100
price: 512.00 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry
citations: 120
reactivity: human, mouse, rat, bovine
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry
citations: 120
Rockland's Affinity Purified anti-Collagen I antibody was used at a 1:100 dilution to detect distal tubules in normal kidney tissue. Note the absence of staining of glomeruli. The antibody was reacted with antibody for 4 hours at room temperature followed by the addition of secondary antibody and substrate reaction. Tissue was formalin-fixed and paraffin embedded. No antigen retrieval was performed.
quantity: 500
price: https://www.rockland.com/search/?searchString=600-401-103-0.5
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry
citations: 2
reactivity: human, mouse, rat, bovine
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry
citations: 2
domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry
reactivity: human, mouse, rat, bovine
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry