domestic rabbit polyclonal
reactivity: human, mouse, bovine
application: western blot, ELISA, immunoprecipitation
reactivity: human, mouse, bovine
application: western blot, ELISA, immunoprecipitation
Rockland biotin conjugated anti adenosine deaminase (200-406-140) was used to detect adenosine deaminase in mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions. The antibody was also used to detect purified Adenosine Deaminase (right, Lane 2), and endogenous Adenosine Deaminase in whole cell lysate from Jurkat and Raji cells (1:1 mixture, lane 3,) as well as Mouse Pancreas and Liver (1:1 mixture, lane 4). Lysates were run on 4-20% gel 140V under reducing conditions, transferred for 30 minutes at 100 V and blocked with 3% Fish Gel (left) or 3% BSA. Blot was incubated with 200-406-140 lot 5628 (1:5K in TBS, ON 4°C), washed 3X in TBS and incubated for 30 minutes with Dylight 488 conjugated Streptavidin (S000-41 lot 20833 1:5K in MB-070). Blot was imaged with the Biorad VersaDoc imaging system.
quantity: 2 mL
price:
to the supplier
domestic rabbit polyclonal
reactivity: bovine
application: western blot, ELISA, immunoprecipitation
reactivity: bovine
application: western blot, ELISA, immunoprecipitation
Rockland biotin conjugated anti adenosine deaminase (200-406-140) was used to detect adenosine deaminase in mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions. The antibody was also used to detect purified Adenosine Deaminase (right, Lane 2), and endogenous Adenosine Deaminase in whole cell lysate from Jurkat and Raji cells (1:1 mixture, lane 3,) as well as Mouse Pancreas and Liver (1:1 mixture, lane 4). Lysates were run on 4-20% gel 140V under reducing conditions, transferred for 30 minutes at 100 V and blocked with 3% Fish Gel (left) or 3% BSA. Blot was incubated with 200-406-140 lot 5628 (1:5K in TBS, ON 4°C), washed 3X in TBS and incubated for 30 minutes with Dylight 488 conjugated Streptavidin (S000-41 lot 20833 1:5K in MB-070). Blot was imaged with the Biorad VersaDoc imaging system.
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: bovine
conjugate: biotin
application: western blot, ELISA, immunoprecipitation
reactivity: bovine
conjugate: biotin
application: western blot, ELISA, immunoprecipitation
Rockland biotin conjugated anti adenosine deaminase (200-406-140) was used to detect adenosine deaminase in mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions. The antibody was also used to detect purified Adenosine Deaminase (right, Lane 2), and endogenous Adenosine Deaminase in whole cell lysate from Jurkat and Raji cells (1:1 mixture, lane 3,) as well as Mouse Pancreas and Liver (1:1 mixture, lane 4). Lysates were run on 4-20% gel 140V under reducing conditions, transferred for 30 minutes at 100 V and blocked with 3% Fish Gel (left) or 3% BSA. Blot was incubated with 200-406-140 lot 5628 (1:5K in TBS, ON 4°C), washed 3X in TBS and incubated for 30 minutes with Dylight 488 conjugated Streptavidin (S000-41 lot 20833 1:5K in MB-070). Blot was imaged with the Biorad VersaDoc imaging system.
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: bovine
conjugate: HRP
application: western blot, ELISA, immunoprecipitation
reactivity: bovine
conjugate: HRP
application: western blot, ELISA, immunoprecipitation