mouse monoclonal (99D)
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry
Flow Cytometry of Mouse Anti-BIN1 Antibody. Cells: C2C12 cells. Stimulation: none. Primary antibody: Anti-IgD (control), Anti-BIN-1 Antibody (99D clone). Secondary antibody: Biotin mouse secondary antibody at 1:10,000 for 45 min at RT and streptavidin PE at 1:5,000 for 30 min at RT.
quantity: 100 µg
price:
to the supplier
mouse monoclonal (99F)
reactivity: human, mouse
application: western blot, ELISA, immunoprecipitation
reactivity: human, mouse
application: western blot, ELISA, immunoprecipitation
Immunoprecipitation of Mouse anti-BIN1 antibody. Immunoprecipitates were separated by SDS-PAGE and visualized by fluorography. The differentiation-associated (Exon 10+) form of Bin1 is recognized by using Bin1 (Exon 10 specific, 99F) monoclonal antibody. Lane 1: BIN 1 Lane 2: none. Load: 35 µg per lane. Primary antibody: BIN-1 (Exon 10 specific, 99F) monoclonal antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 64.7 kDa, ~65 kDa for BIN-1. Other band(s): none.
quantity: 100 µg
price:
to the supplier
mouse monoclonal (2F11)
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
Immunohistochemistry of Mouse Anti-BIN1 antibody. Tissue: skeletal muscle from BIN-1 wild type (+/+) and null (-/-) mouse. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: BIN-1 antibody for 1hr at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN1 as precipitated brown signal.
quantity: 100 µg
price:
to the supplier
mouse monoclonal (12A)
reactivity: human
application: western blot, ELISA
reactivity: human
application: western blot, ELISA
Western Blot of Mouse Anti-BIN-1 Antibody. Lane 1: LNCap. Lane 2: WM1341D. Lane 3: WM164. Lane 4: WM983A. Lane 5: WM793. Lane 6: WM239A. Lane 7: WM35. Load: 35 µg per lane. Primary antibody: BIN-1 antibody (Exon 12A specific) at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
quantity: 100 µg
price:
to the supplier