Pdcd4 Antibody
Rockland Immunochemicals
catalog: 600-401-965

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, reverse phase protein lysate microarray, other
citations: 6

Western blot using Rockland's affinity purified anti-Pdcd4 antibody shows detection of a band ~52 kDa in size corresponding to Pdcd4 (arrowhead). Lane 1 contains recombinant Pdcd4. Lane 2 contains 293 HEK cells treated with TPA and MG132. The anti-Pdcd4 antibody was used at a 1: 5,000 dilution. Personal Communication. M Young & A Jansen, NCI, Bethesda, MD.
quantity: 100 µg
price:
to the supplier

Pdcd4 phospho S457 Antibody
Rockland Immunochemicals
catalog: 200-301-B17

mouse monoclonal (9G6)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry
citations: 2

quantity: 1 mg
price:
to the supplier

Ready-To-Use Pdcd4 Antibody
Rockland Immunochemicals
catalog: 600-401-965-RTU

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
citations: 3

Western Blot Results of Ready to Use Rabbit Anti-Pdcd4 Antibody. Lane 1: Opal Pre-stained Molecular Weight Ladder (p/n MB-210-0500). Lane 2: HEK293T WC lysate. Lane 3: HeLa WC Lysate (p/n W09-000-364). Load: 10µL. Primary Antibody: Ready to Use Rabbit Anti-Pdcd4 Antibody 1:1000 overnight at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP (p/n 611-103-122) at 1:70,000 for 30 min RT. Block: BlockOut Buffer (p/n MB-073). Expect: ~52 kDa. Observed: ~35kDa.
quantity: 100 µL
price:
to the supplier

Pdcd4 phospho S457 Antibody
Rockland Immunochemicals
catalog: 600-401-964

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Rockland's affinity purified anti-Pdcd4 pS457 antibody was used at 1.25 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of human breast epithelial cells at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
quantity: 100 µg
price:
to the supplier

Pdcd4 phospho S457 Antibody
Rockland Immunochemicals
catalog: 200-301-964

mouse monoclonal (9G6)
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry
citations: 1

Rockland Antibody 200-301-964 has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The antibody was serially diluted and tested at a range of concentrations on at least 22 different human formalin-fixed, paraffin archival tissues, and positive and negative tissues were scored and compared to the published literature on the expression and function of the gene. A representative image from positively stained small intestine shows the localization of the anti Pdcd4 antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Image provided courtesy of LifeSpan Biosciences, Seattle, WA
quantity: 100 µg
price:
to the supplier

Pdcd4 phospho S457 Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 200-306-964

mouse monoclonal (9G6)
reactivity: human, mouse, rat
conjugate: biotin
application: western blot, ELISA, immunoprecipitation

ELISA of Mouse anti-Pdcd4 phospho S457 Biotin Conjugated antibody. Antigen: BSA conjugates of Pdcd4 phospho S457 and Pdcd4 non-phospho S457. Coating amount: 0.1 µg per well. Primary antibody: Pdcd4 phospho S457 Biotin Conjugated antibody at 5 µg/mL. Dilution series: 3-fold. Mid-point concentration: 5 ng/mL Pdcd4 phospho S457 Biotin Conjugated antibody. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE-0100)
quantity: 50 µg
price:
to the supplier