rabbit polyclonal
reactivity: human, mouse, rat, dog
application: western blot, ELISA
reactivity: human, mouse, rat, dog
application: western blot, ELISA
Western blot using Rockland's Affinity Purified anti-CaM Kinase II antibody shows detection of a band ~54 kDa corresponding to human alpha CaM Kinase II (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Approximately 35 ?g of a mouse brain whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CaM Kinase II was similarly detected on lysates from rat brain (not shown). After blocking the membrane was probed with the primary antibody diluted to 1:1,500 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
quantity: 100 µg
price: 369 USD
to the supplier
rabbit polyclonal
reactivity: mouse, rat
application: western blot, ELISA
reactivity: mouse, rat
application: western blot, ELISA
Western Blot of Rabbit anti- CaM Kinase II Thr286 Antibody. Lane 1: rat brain lysate. Lane 2: rat brain lysate incubated in ?-Ptase (1200 units for 30 min). Load: 10 µg per lane. Primary antibody: CaM Kinase II Thr286 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~50kDa ?- and the ~60kDa for ?-CaM Kinase II phosphorylated at Thr286. Other band(s): none.
quantity: 100 µL
price: 366 USD
to the supplier
mouse monoclonal (6G9)
reactivity: mouse, rat, cow
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
reactivity: mouse, rat, cow
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
Immunofluorescence Microscopy of mouse anti-CaMKII antibody. Tissue: Hippocampal neurons. Fixation: N/A. Primary antibody: anti-CaMKII for 1h at RT. Seondary antibody: Fluorescein mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: Cytoplasm. Staining: CaMKII as precipiated ed signal.
quantity: 100 µg
price: 369 USD
to the supplier
mouse monoclonal (22B1)
reactivity: rat
application: western blot, ELISA, immunoprecipitation
reactivity: rat
application: western blot, ELISA, immunoprecipitation
Immunofluorescence Microscopy of mouse Anti-CaMKII antibody. Tissue: Backskin section of transgenic mice. Fixation: Paraffin-embedded. Primary antibody: anti-CaMKII for 1h at RT. Seondary antibody: Peroxidase mouse secondary at 1:10,000 for 45 min at RT. Localization: Cell junction. Staining: Calreticulin as precipiated green signal.
quantity: 100 µg
price: 369 USD
to the supplier