HA Epitope Tag Antibody
Rockland Immunochemicals
catalog: 600-401-384

domestic rabbit polyclonal
application: western blot, ELISA, immunohistochemistry
citations: 45

Rockland's Affinity Purified anti-HA epitope tag polyclonal antibody detects HA tagged recombinant proteins by IHC on formalin fixed paraffin embedded tissue. Arrowheads point to expression of HA tagged proteins in endothelial cells of mouse aorta. Sections of 4 µm were prepared from representative paraffin blocks. Sections were then deparaffinized and rehydrated with xylene and alcohol. Citrate buffer antigen retrieval was performed for 30 min in a boiling jar. Anti-HA was diluted in blocking buffer at 1:2,000 and reacted at 4° C overnight followed by signal detection using horseradish peroxidase with DAB as the chromogenic substrate. Tissue was counterstained with Mayer's hematoxylin. Personal Communication, Behzad Yeganeh,U. Manitoba, Winnipeg, Canada.
quantity: 100
price: 335.00 USD
to the supplier

HA Epitope Tag Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 600-406-384

domestic rabbit polyclonal
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

Anti-HA epitope tag polyclonal antibody detects HA tagged recombinant proteins by western blot. Polyclonal rabbit-anti-HA epitope tag at a 1:10,000 dilution was used to detect 1.0 ug of recombinant transcription factor protein containing the HA epitope tag. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with Rockland's anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:4000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results
quantity: 100 µg
price:
to the supplier

HA Epitope Tag Antibody Peroxidase Conjugated
Rockland Immunochemicals
catalog: 600-403-384

domestic rabbit polyclonal
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

quantity: 100 µg
price:
to the supplier

HA Epitope Tag Antibody Dylight™ 488 Conjugated
Rockland Immunochemicals
catalog: 600-441-384

domestic rabbit polyclonal
conjugate: DyLight488
application: western blot

Properties of Dylight™ Conjugates.

Western Blot of Rabbit anti-HA DyLight™ 488 Conjugated Antibody. Lane 1: 12 Epitope Tag Protein Marker Lysate. Lane 2: None. Load: 10 uL. Primary antibody: None. Secondary antibody: DyLight™ 488 rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 69 kDa, 69 kDa for HA. Other band(s): None.
quantity: 100 µg
price:
to the supplier

HA Epitope Tag Antibody Dylight™ 549 Conjugated
Rockland Immunochemicals
catalog: 600-442-384

domestic rabbit polyclonal
conjugate: DyLight549
application: western blot

quantity: 100 µg
price:
to the supplier

HA Epitope Tag Antibody Dylight™ 649 Conjugated
Rockland Immunochemicals
catalog: 600-443-384

domestic rabbit polyclonal
conjugate: DyLight649
application: western blot

DyLight™ dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight™ 549 conjugate. Anti-TNFa was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.
quantity: 100 µg
price:
to the supplier

HA Epitope Tag Antibody Dylight™ 680 Conjugated
Rockland Immunochemicals
catalog: 600-444-384

domestic rabbit polyclonal
conjugate: DyLight680
application: western blot

quantity: 100 µg
price:
to the supplier

HA Epitope Tag Antibody Dylight™ 800 Conjugated
Rockland Immunochemicals
catalog: 600-445-384

domestic rabbit polyclonal
conjugate: DyLight800
application: western blot

quantity: 100 µg
price:
to the supplier

HA Epitope Tag Antibody Dylight™ 405 Conjugated
Rockland Immunochemicals
catalog: 600-446-384

domestic rabbit polyclonal
conjugate: DyLight 405
application: western blot

Properties of DyLight™ Fluorescent Dyes.
quantity: 100 µg
price:
to the supplier