rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry
Western blot using Rockland's affinity purified anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution, overnight at 4° C. Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.
quantity: 100 µg
price: 369 USD
to the supplier
mouse monoclonal (5F6.F1)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry
Immunohistochemistry using Rockland's Anti-Stat5 pY694 monoclonal antibody shows detection of phosphorylated Stat5pY694 in human breast tissue (40X). The antibody was used a dilution to 20 µg/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.
quantity: 100 µg
price: 369 USD
to the supplier
mouse monoclonal (5F6.F1)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry
Immunohistochemistry using Rockland Immunochemicals Anti-STAT5 pY694 monoclonal antibody shows detection of phosphorylated STAT5 pY694 in human breast tissue (40X). The antibody was used a dilution to 20 µg/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.
quantity: 1 mg
price: 2265 USD
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA
reactivity: human, mouse, rat
application: western blot, ELISA
Western blot using Rockland's affinity purified anti-STAT5 antibody shows detection of Stat5 protein in HeLa cell extract (arrowhead). Primary antibody was used at a 1:600 dilution reacted overnight at 4°C. Secondary antibody was used at 1:20,000. Molecular weight estimation was made by comparison to prestained MW markers. Identity of the upper and lower bands is unknown. Personal communication, Luanne Lukes, NCI, Bethesda, MD
quantity: 100 µg
price: 369 USD
to the supplier