IL-27/p28 Antibody
Rockland Immunochemicals
catalog: 210-401-B54

domestic rabbit polyclonal
reactivity: mouse
application: western blot, ELISA

Detection of recombinant IL27/p28 protein by Rockland's anti-Mouse IL-27/p28 antibody. Recombinant mouse IL27/p28 was loaded on to an SDS-PAGE gel at 0.25 µg and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
quantity: 100 µg
price:
to the supplier

IL-27/p28 Antibody Peroxidase Conjugated
Rockland Immunochemicals
catalog: 210-403-B54

domestic rabbit polyclonal
reactivity: mouse
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

Recombinant mouse IL27/p28 was loaded at 0.25 µg. The blot was blocked with 1% BSA in TBST for 30 min at RT. Blot was incubated with HRP rabbit anti-Mouse IL-27/p28 in 1% BSA/TBST at 1:5,000 for 30 min at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP.
quantity: 100 µg
price:
to the supplier

IL-27/p28 Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 210-406-B54

domestic rabbit polyclonal
reactivity: mouse
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

quantity: 100 µg
price:
to the supplier

Mouse IL-27/p28 Antibody
Rockland Immunochemicals
catalog: 210-501-B54

rat monoclonal (3H12.F10)
reactivity: mouse
application: western blot, ELISA, flow cytometry

Mouse peritoneal macrophages were grown in culture for 24 hours, stimulated with 10ng/mL IFN? and 1ug/mL LPS for 14 hours and incubated for 4 hours with Bredfeldin A. Cells were harvested, washed, aliquoted 1x106 cells per sample, and fixed and permeabilized according to a standard protocol. Samples were stained with biotinylated primary anti-mouse p28 antibody at (0.1 - 10ug/mL primary antibody alongside negative controls of unstimulated cells and isotype controls. Cells were stained with 0.25ug/mL rat anti-mouse CD107b conjugated Alexa Fluor 647 and PHYCOERYTHRIN Conjugated secondary at 1:100 and analyzed by flow cytometry. Stimulated cells showed increase PE staining (horizontal axis) when compared with unstimulated cells.
quantity: 100 µg
price:
to the supplier

IL-27 Antibody
Rockland Immunochemicals
catalog: 600-401-BW1

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

quantity: 100 µg
price:
to the supplier

IL-27 Antibody
Rockland Immunochemicals
catalog: 600-401-BW2

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

quantity: 100 µg
price:
to the supplier