AKT phospho S473 Antibody
Rockland Immunochemicals
catalog: 200-301-268

mouse monoclonal (17F6.B11)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry
citations: 3

Immunohistochemistry of Mouse anti-AKT pS473 antibody. Tissue: human prostate tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT pS473 antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT. Localization: AKT pS473 is nuclear and occasionally cytoplasmic. Staining: AKT pS473 as precipitated red signal with hematoxylin purple nuclear counterstain.
quantity: 100 µg
price:
to the supplier

AKT Antibody
Rockland Immunochemicals
catalog: 100-401-401

domestic rabbit polyclonal
reactivity: human, mouse, rat, chicken
application: western blot, ELISA, immunohistochemistry, flow cytometry
citations: 1

AKT Metabolic Pathway
quantity: 200
price: https://www.rockland.com/search/?searchString=100-401-401
to the supplier

AKT phospho S473 Antibody
Rockland Immunochemicals
catalog: 600-401-268

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry
citations: 1

Immunohistochemistry of Rabbit Anti-Akt pS473 antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Akt pS473 antibody at 100 dilution for 1 h at RT. Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT. Localization: Akt pS473 is nuclear and occasionally cytoplasmic.
quantity: 100 µg
price:
to the supplier

AKT Antibody
Rockland Immunochemicals
catalog: 200-301-401

mouse monoclonal (14E5.16C8.25F6)
reactivity: human, mouse, rat, chimpanzee
application: western blot, ELISA, immunohistochemistry, flow cytometry
citations: 1

Western Blot of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates. Lane 2: PDGF stimulated NIH/3T3 cell lysates. Load: 10 µg per lane. Primary antibody: AKT antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG (p/n 610-103-121) was used at a 1:40,000 dilution for 1 h at 4° C with FemtoMax™ enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Block: 5% BLOTTO (p/n B501-0500 in TBS for 2h at RT. Observed size: ~56 kDa for AKT. Other band(s): none.

Western Blot of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates. Lane 2: PDGF stimulated NIH/3T3 cell lysates. Load: 10 µg per lane. Primary antibody: AKT antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG (p/n 610-103-121) was used at a 1:40,000 dilution for 1 h at 4° C with FemtoMax™ enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Block: 5% BLOTTO (p/n B501-0500 in TBS for 2h at RT. Observed size: ~56 kDa for AKT. Other band(s): none.
quantity: 100 µg
price:
to the supplier

AKT phospho T308 Antibody
Rockland Immunochemicals
catalog: 600-401-269

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, flow cytometry

Western Blot of Rabbit anti-Akt phospho T308 antibody. Lane 1: GST tagged AKT1 un-active recombinant protein. Lane 2: GST tagged AKT1 active recombinant protein. Load: 50 ng per lane. Primary antibody: Akt phospho T308 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Other band(s): none.
quantity: 100 µg
price:
to the supplier

AKT phospho S473 Dylight 488 Conjugated Antibody
Rockland Immunochemicals
catalog: 200-341-268

mouse monoclonal (17F6.B11)
reactivity: human, mouse, rat
conjugate: DyLight488
application: western blot, ELISA, immunohistochemistry, flow cytometry

Properties of DyLight™ Fluorescent Dyes.
quantity: 100 µg
price:
to the supplier

AKT phospho T308 Dylight 488 Conjugated Antibody
Rockland Immunochemicals
catalog: 200-341-269

mouse monoclonal (18F3.H11)
reactivity: human, mouse, rat
conjugate: DyLight488
application: western blot, ELISA, flow cytometry

DyLight™ dye 488#DyLight™ dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image.  DyLight™ dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods.  This image shows anti-histone detection using a DyLight™ 488 conjugate (green).  Anti-Tubulin was detected using a DyLight™ 549 conjugate (red).  Nuclei were counter-stained using DAPI (blue).  The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).
quantity: 100 µg
price:
to the supplier

AKT phospho S473 Dylight 549 Conjugated Antibody
Rockland Immunochemicals
catalog: 200-342-268

mouse monoclonal (17F6.B11)
reactivity: human, mouse, rat
conjugate: DyLight549
application: western blot, ELISA, flow cytometry

DyLight™ dyes can be used for two-color western blot detection with low background and high signal. Anti-tubulin was detected using a DyLight™ 549 conjugate. Anti-TNFa was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.
quantity: 100 µg
price:
to the supplier

AKT phospho T308 Dylight 549 Conjugated Antibody
Rockland Immunochemicals
catalog: 200-342-269

mouse monoclonal (18F3.H11)
reactivity: human, mouse, rat
conjugate: DyLight549
application: western blot, ELISA, immunohistochemistry, flow cytometry

DyLight™ dyes can be used for two-color western blot detection with low background and high signal. Anti-tubulin was detected using a DyLight™ 549 conjugate. Anti-TNFa was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.
quantity: 100 µg
price:
to the supplier

AKT phospho S473 Dylight 649 Conjugated
Rockland Immunochemicals
catalog: 200-343-268

mouse monoclonal (17F6.B11)
reactivity: human, mouse, rat
conjugate: DyLight649
application: western blot, ELISA, flow cytometry

DyLight dyes can be used for two-color Western Blot detection with low background and high signal.  Anti-tubulin was detected using a DyLight™ 549 conjugate.  Anti-TNFa was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.
quantity: 100 µg
price:
to the supplier

AKT phospho T308 Dylight 649 Conjugated Antibody
Rockland Immunochemicals
catalog: 200-343-269

mouse monoclonal (18F3.H11)
reactivity: human, mouse, rat
conjugate: DyLight649
application: western blot, ELISA, flow cytometry

DyLight™ dyes can be used for two-color Western Blot detection with low background and high signal.  Anti-tubulin was detected using a DyLight™ 549 conjugate.  Anti-TNFa was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.
quantity: 100 µg
price:
to the supplier

AKT phospho S473 Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 200-306-268

mouse monoclonal (17F6.B11)
reactivity: human, mouse, rat
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry

Immunohistochemistry of mouse anti AKT phospho S473 biotin conjugated at 40X (left) with negative control (right) Tissue: prostate Fixation: FFPE buffered formalin 10% conc Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 20 µg/mL for 1 h at RT Secondary antibody: Streptavidin Conj. HRP 10 ug/ml Localization: nuclear and occasionally cytoplasmic Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
quantity: 50 µg
price:
to the supplier

AKT phospho T308 Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 200-306-269

mouse monoclonal (18F3.H11)
reactivity: human, mouse, rat
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry

Western Blot of Mouse anti-Akt phospho T308 Biotin Conjugated antibody. Lane 1: GST tagged AKT1 active recombinant protein. Lane 2: GST tagged AKT1 un-active recombinant protein. Load: 25 ng per lane. Primary antibody: Akt phospho T308 Biotin Conjugated antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 79 kDa, 79 kDa for Akt phospho T308. Other band(s): none
quantity: 50 µg
price:
to the supplier

AKT phospho S473 ATTO594 Conjugated Antibody
Rockland Immunochemicals
catalog: 200-355-268

mouse monoclonal (17F6.B11)
reactivity: human, mouse, rat
conjugate: ATTO 594
application: western blot, ELISA, flow cytometry

ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.

Western Blot of Mouse anti-AKT pS473 antibody Atto 594 Conjugated. Lane 1: GST Tagged AKT 1 Active Recombinant Protein. Lane 2: None. Load: 25 ng per lane. Primary antibody: None. Secondary antibody: Atto 594 mouse secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: ~100 kDa, ~100 kDa for AKT pS473. Other band(s): None
quantity: 100 µg
price:
to the supplier

AKT1 FITC Antibody
Rockland Immunochemicals
catalog: 200-302-I51

mouse monoclonal (14E5.A2.B2.H9)
reactivity: human, mouse, rat
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, flow cytometry

quantity: 50 µg
price:
to the supplier

AKT1 FITC Antibody
Rockland Immunochemicals
catalog: 200-302-J34

mouse monoclonal (5E5.F5.D7)
reactivity: human, mouse, rat
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, flow cytometry

quantity: 50 µg
price:
to the supplier

AKT1 BIOTIN Antibody
Rockland Immunochemicals
catalog: 200-306-I51

mouse monoclonal (14E5.A2.B2.H9)
reactivity: human, mouse, rat
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, flow cytometry

quantity: 50 µg
price:
to the supplier

AKT1 BIOTIN Antibody
Rockland Immunochemicals
catalog: 200-306-J34

mouse monoclonal (5E5.F5.D7)
reactivity: human, mouse, rat
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, flow cytometry

quantity: 50 µg
price:
to the supplier

AKT1 PE Antibody
Rockland Immunochemicals
catalog: 200-308-I51

mouse monoclonal (14E5.A2.B2.H9)
reactivity: human, mouse, rat
conjugate: RPE
application: western blot, ELISA, immunohistochemistry, flow cytometry

quantity: 50 µg
price:
to the supplier

AKT1 PE Antibody
Rockland Immunochemicals
catalog: 200-308-J34

mouse monoclonal (5E5.F5.D7)
reactivity: human, mouse, rat
conjugate: RPE
application: western blot, ELISA, immunohistochemistry, flow cytometry

quantity: 50 µg
price:
to the supplier

AKT1 APC Antibody
Rockland Immunochemicals
catalog: 200-326-I51

mouse monoclonal (14E5.A2.B2.H9)
reactivity: human, mouse, rat
conjugate: APC
application: western blot, ELISA, immunohistochemistry, flow cytometry

quantity: 50 µg
price:
to the supplier

AKT1 APC Antibody
Rockland Immunochemicals
catalog: 200-326-J34

mouse monoclonal (5E5.F5.D7)
reactivity: human, mouse, rat
conjugate: APC
application: western blot, ELISA, immunohistochemistry, flow cytometry

quantity: 50 µg
price:
to the supplier