NAG-1 Antibody
Rockland Immunochemicals
catalog: 600-401-B07

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry

Immunohistochemistry of Rabbit anti NAG1 antibody (C-terminal specific) at 20X in colon tissue at pH 9 . Negative control of human colon tissue pH9 is shown in background. Tissue: Human Colon
quantity: 100
price: 449.00 USD
to the supplier

NAG-1 Antibody
Rockland Immunochemicals
catalog: 209-401-B88

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

Western blot using Rockland's affinity purified anti-Human NAG-1 antibody shows detection of a 42 kDa band corresponding to purified recombinant fusion protein MBP-human NAG-1.   Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Primary antibody was used at 1:1000 in 1% BSA-TBST overnight at 4°C. DyLight649™ Goat Anti-Rabbit IgG secondary antibody (p/n 611-143-122) was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™ 4000MP Imaging System. Other detection systems will yield similar results.
quantity: 100 µg
price:
to the supplier

NAG-1 Antibody Peroxidase Conjugated
Rockland Immunochemicals
catalog: 209-403-B88

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA

quantity: 100 µg
price:
to the supplier

NAG-1 Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 209-406-B88

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

quantity: 100 µg
price:
to the supplier

NAG-1 Antibody
Rockland Immunochemicals
catalog: 210-401-B68

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

Western blot using Rockland's affinity purified anti-Mouse NAG-1/GDF15 antibody. The blot shows detection of recombinant MBP-NAG-1 fusion protein (60 kDa) purified from E.coli (lane 1); yeast cell lysate expressing SUMO-mouse NAG-1 (42 kDa) (lane 2), and R&D human NAG-1 monomer purified from CHO-K1 cells (14 kDa) (lane 3). All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution in TBS containg 1% BSA and 0.2% Tween, and reacted overnight at 4°C. Nag-1 was detected using a 1:40,000 dilution of peroxidase conjugated Gt-a-Rabbit antibody (611-103-122) in Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™ 4000MP Imaging System. Other detection systems will yield similar results.
quantity: 100 µg
price:
to the supplier

NAG-1 Antibody Peroxidase Conjugated
Rockland Immunochemicals
catalog: 210-403-B68

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

quantity: 100 µg
price:
to the supplier

NAG-1 Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 210-406-B68

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

quantity: 100 µg
price:
to the supplier

NAG-1 Antibody Peroxidase Conjugated
Rockland Immunochemicals
catalog: 600-403-B07

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

Western blot using Rockland's affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electro-phoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting.
quantity: 100 µg
price:
to the supplier

NAG-1 Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 600-406-B07

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

Western blot using Rockland's affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electro-phoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting.
quantity: 100 µg
price:
to the supplier

Nag-1 Antibody
Rockland Immunochemicals
catalog: 610-401-B87

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

Western blot using Rockland's affinity purified anti-mouse NAG-1/GDF15 antibody. The blot shows detection of recombinant MBP-NAG-1 fusion protein (60 kDa) purified from E.coli (lane 1); yeast cell lysate expressing SUMO-mouse NAG-1 (42 kDa) (lane 2), and R&D human NAG-1 monomer purified from CHO-K1 cells (14 kDa) (lane 3). All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution in TBS containg 1% BSA and 0.2% Tween, and reacted overnight at 4°C. Nag-1 was detected using a 1:40,000 dilution of peroxidase conjugated Gt-a-Rabbit antibody (611-103-122) in Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™ 4000MP Imaging System. Other detection systems will yield similar results.
quantity: 100 µg
price:
to the supplier