mouse monoclonal (70B1413.1)
reactivity: human, mouse
application: western blot
reactivity: human, mouse
application: western blot
Western Blot of Mouse Anti-ATF6 antibody. Lane 1: 293 cells transfected with full-length ATF6. Lane 2: 293 cells transfected with partial length ATF6. Lane 3: Untransfected 293 cells. Load: 30 µg per lane. Primary antibody: ATF6 antibody at 4 µg/ml for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~90 kDa for ATF6. Other band(s): none.
Western Blot of Mouse Anti-ATF6 antibody. Lane A: NIH3T3 lysate. Load: 30 µg per lane. Primary antibody: ATF6 antibody at 3 µg/ml for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~90 kDa for ATF6 antibody. Other band(s): possible breakdown/cleavage product.
quantity: 100 µg
price:
to the supplier
domestic goat polyclonal
reactivity: human
application: ELISA
reactivity: human
application: ELISA
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA
reactivity: human, mouse
application: western blot, ELISA
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry
Immunofluorescence Microscopy of Rabbit anti-ATF6 antibody. Tissue: U-251 cells p/n (W09-001-GY4). Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: ATF6 antibody at 10 µg/mL overnight at 4⁰C. Secondary antibody: Anti-RABBIT IgG DyLight™ 488 Conjugated Preadsorbed (p/n 611-741-127) at 5 ug/ml for 2 h at RT. Localization: ATF6 is cell membrane and cytoplasmic. Staining: ATF6 as green fluorescent signal with DAPI (blue) nuclear counterstain.
quantity: 100 µg
price:
to the supplier