product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Pancreatic Islet Lysate Antibody
catalog :
214-A01-118
quantity :
500 µg
clonality :
polyclonal
host :
dogs
conjugate :
nonconjugated
reactivity :
pigs
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
214-A01-118
Name :
Anti-PANCREATIC ISLET LYSATE (Porcine Pancreas) (DOG) Antibody - 214-A01-118
Display Name :
Pancreatic Islet Lysate Antibody
Application Note :
This product has been assayed against 1.0 ug of Pancreatic Islet Lysate [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) code #604-1302 and o-Phenylenediamine (OPD) as a substrate.  A working dilution of 1:50 to 1:100 is suggested for this product.  This product has been assayed against 1.5 ug of Pancreatic Islet Lysate [Porcine Pancreas] per lane by immunoblot (western) under reducing conditions of SDS-PAGE prior to transfer to nitrocellulose and detection using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) code #604-1302 and Diaminobenzidine (DAB) as a substrate.  A working dilution of 1:50 to 1:200 is suggested for this product.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Conjugation :
(None)
Size :
500 µg
Default Unit :
µg
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Gene Name :
PPY
Host Animal :
Dog
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog Serum.  Cross reactivity was determined by immunoblot (western) to be minimal against porcine kidney and porcine liver cell lysates.
Species Reactivity :
porcine
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
IgG fraction of Anti-pancreatic islet lysate, Dog Anti-Pancreatic lysate (porcine pancreas), Anti-pancreatic lysate produced in dog, dog anti-Pancreatic Islet Lysate Antibody, canine anti-Pancreatic Islet Lysate Antibody
Target Species :
Swine
Background :
Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions. It may be used as a marker of the viability of xenotransplanted fetal panreatic tissue in the immediate post-transplant period.
Immunogen Type :
Native Protein
Low Endotoxin :
No
Sample Size :
No
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
P01300
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.