TNF alpha Antibody | Rockland Immunochemicals 214-401-306 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

TNF alpha Antibody

catalog :

214-401-306

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

pigs

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

214-401-306

Name :

Anti-Swine TNFα (RABBIT) Antibody - 214-401-306

Display Name :

TNF alpha Antibody

Application Note :

This protein A purified antibody is suitable for ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 16.9 kDa in size corresponding to swine TNFα by western blotting in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:2,000 - 1:2,500

Immunohistochemistry Dilution :

User Optimized

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

TNF

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to 154 amino acids of mature swine TNFα.

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This product was Protein-A purified from monospecific antiserum by chromatography. It is specific for swine TNFα protein. A BLAST analysis was used to suggest cross-reactivity with TNFα from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against TNFα from baboon based on 90% homology. Cross-reactivity with TNFα from sources other than swine has not been determined.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

100 µL

Species Reactivity :

Swine

Storage :

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-TNF-A antibody, rabbit anti-TNFα antibody, rabbit anti-TNF alpha antibody, cachexin, cachectin, tumor necrosis factor-alpha, TNFSF2, TNFSF1A

Target Species :

Swine

Background :

Tumor necrosis factor-alpha (TNFα) in humans is a pleiotropic inflammatory cytokine. It is expressed by many different stimulated cell types including monocytes, endothelial cells, fibroblasts and both T and B-lymphocytes, but especially by macrophages. It affects most organs. The production of TNF alpha is induced by IL-1, PDGF, IFN-beta, NGF, Oncostatin M and certain fungal, viral, and parasitic invasions. Bacterial lipopolysaccharide is an especially potent stimulus. The primary role of TNFα is in the regulation of immune cells. TNF α mediates septic shock in response to infection. It initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophages and neutrophils. TNFα secreted by macrophages causes blood clotting which helps contain infection. TNFα is also able to induce apoptotic cell death or inflammation, and to inhibit viral replication. It possesses growth stimulating, inhibitory, and self regulatory properties. Dysregulation of TNFα production has been implicated in a variety of human diseases, as well as cancer. The presence of TNFα is responsible for diverse immunomodulatory and toxic effects. For instance, TNFα induces neutrophil proliferation during inflammation, but it also induces neutrophil apoptosis upon binding to the TNF-R55 receptor. TNFα causes necrosis of some types of tumors, but promotes growth of other types. Low levels may aid in maintaining homeostasis by regulating the body's circadian rhythm, and may promote remodeling or replacement of injured and senescent tissue by stimulating fibroblast growth. High levels of TNFα correlate with increased risk of mortality.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P23563

NCBI :

NP_999187

Primary Image Name :

Anti-Swine TNFa Antibody - Western Blot

more info or order :

Rockland Immunochemicals product webpage