IDO1 Antibody | Rockland Immunochemicals 210-301-E58S product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

IDO1 Antibody

catalog :

210-301-E58S

quantity :

25 µL

price :

99.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2E2.6

reactivity :

mouse

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry

product information

Catalog Number :

210-301-E58S

Name :

Anti-IDO1 (MOUSE) Monoclonal Antibody - 210-301-E58S

Display Name :

IDO1 Antibody

Application Note :

Anti-IDO1 antibody has been tested for use in ELISA, Western Blot, IF, IHC, and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

2E2.6

Concentration Value :

0.95 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

25 µL

Default Unit :

ELISA Dilution :

1:5000-1:50000

Flow Cytometry Dilution :

0.5-1x10^6 cells

Immunohistochemistry Dilution :

User Optimized

IF Microscopy Dilution :

1:50-1:100

Immunoprecipitation Dilution :

10-100 µL

Western Blot Dilution :

1:500-1:1500

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

Ido1

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

IDO1 antibody was produced in mouse by repeated immunizations with mouse recombinant IDO1 protein followed by hybridoma development.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-IDO1 antibody was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. IDO1 antibody is specific for mouse IDO1 protein. Mouse IDO1 does not react with human tissues. Cross-reactivity with IDO1 from other sources has not been determined.

Species Reactivity :

Mouse

Storage :

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Subclass :

IgG1

Synonyms :

mouse anti-IDO1 antibody, Ido, Indo, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase, Ido 1, Ido-1, IDO1 antibody, mouse anti-IDO-1 antibody

Target Species :

Mouse

Background :

Anti-IDO-1 antibody recognizes indoleamine 2, 3-dioxygenase1 (IDO1) is a 41-42 kD intracellular enzyme that catabolizes tryptophan into kynurenine. IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO1 effects on immune suppression are due to decreased tryptophan availability and the generation of tryptophan metabolites, resulting in negative effects on T lymphocytes, including proliferation, function and survival. IDO1 may be involved in the suppression of the immune response to tumors, and blocking the IDO1 pathway may be a potential target for immuno and cancer therapy. IDO1 is expressed in a wide variety of tissues and can be upregulated by interferon gamma and other inflammatory cytokines.

General Reference :

Mellor AL., et al. (2004) Nat Rev Immunol 4:762-774. Godin-Ethier J., et al. (2011) Clin Cancer Res 17:6985-6991. Mellor AL., et al. (2002) J Immunol 168:3771-3776. Frumento G., et al. (2002) J Exp Med 196:459-468. Muller AJ., et al. (2005) Nat Med 11:312-319. Lob S., et al. (2009) Nat Rev Cancer 9:445-452. Uyttenhove C., et al. (2003) Nat Med 9:1269-1274. Daubener w., et al. (1999) Adv Exp Med Biol 467:517-524. Wechsler-Reya, R., D. et al. (1997) J. Biol Chem 272:31453-31458.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Yes

Application Text :

ELISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2019 Price :

99.00 USD

UniProt :

P28776

NCBI :

NP_032350.1

Primary Image Name :

Immunofluorescence - IDO1 Antibody