Human IgG (H&L) Antibody Peroxidase Conjugated | Rockland Immunochemicals 209-4302 product information

product summary

company name :

Rockland Immunochemicals

product type :

secondary antibody

product name :

Human IgG (H&L) Antibody Peroxidase Conjugated

catalog :

209-4302

quantity :

20 mg

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

209-4302

Name :

Anti-HUMAN IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated - 209-4302

Display Name :

Human IgG (H&L) Antibody Peroxidase Conjugated

Application Note :

Secondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.

Buffer :

0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

10.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

HRP

Size :

20 mg

Default Unit :

ELISA Dilution :

1:10,000 - 1:50,000

Immunohistochemistry Dilution :

1:500 - 1:2,500

Western Blot Dilution :

1:1,000 - 1:10,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Human IgG whole molecule

Packing Type :

Ambient

Physical State :

Lyophilized

Purity and Specificity :

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG and Human Serum.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

2.0 mL

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-Human IgG peroxidase Conjugated Antibody, rabbit anti-Human IgG HRP Conjugated Antibody, rabbit anti-Human IgG Antibody peroxidase Conjugation

Target Species :

Human

Background :

Anti-Human IgG (H&L) Peroxidase generated in rabbit detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present. It is a protein complex composed of four peptide chains — two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75% of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Low Endotoxin :

Sample Size :

Secondary Antibody Specificity :

IgG (H&L)

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Secondary Antibodies

Conjugation Name :

Peroxidase (Horseradish)

more info or order :

Rockland Immunochemicals product webpage