IL-3 Antibody Biotin Conjugated | Rockland Immunochemicals 209-406-B92 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

IL-3 Antibody Biotin Conjugated

catalog :

209-406-B92

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

biotin

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

209-406-B92

Name :

Anti-Human IL-3 (RABBIT) Antibody Biotin Conjugated - 209-406-B92

Display Name :

IL-3 Antibody Biotin Conjugated

Application Note :

This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 15 kDa in size corresponding to human IL-3 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

BAC

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:20,000-1:100,000

Immunohistochemistry Dilution :

1:1,000-1:5,000

Western Blot Dilution :

1:2,000-1:10,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

IL3

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Human IL-3 (RABBIT) Antibody Biotin Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-3 protein.

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Biotin Conjugated Anti-Human IL-3 Antibody Biotin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-3 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

100 µL

Species Reactivity :

human

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store Anti-Human IL-3 (RABBIT) Antibody Biotin Conjugated at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-IL-3 biotin conjugated antibody, rabbit anti-Interleukin 3 biotin conjugated antibody, IL3, interleukin-3, Burst promoting activity, Colony stimulating factor, Eosinophil colony stimulating factor, Hematopoietic growth factor, IL-3, Hematopoietic growth factor, Mast cell growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor

Target Species :

Human

Background :

The pleiotropic IL3 (Interleukin 3) is a 15kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Biotin

UniProt :

P08700

NCBI :

NP_000579

Primary Image Name :

WB - IL-3 Antibody Biotin Conjugated

more info or order :

Rockland Immunochemicals product webpage