MIP-1 beta Antibody Peroxidase Conjugated | Rockland Immunochemicals 209-403-B97 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

MIP-1 beta Antibody Peroxidase Conjugated

catalog :

209-403-B97

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

HRP

reactivity :

human

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

209-403-B97

Name :

Anti-Human MIP-1ß (RABBIT) Antibody Peroxidase Conjugated - 209-403-B97

Display Name :

MIP-1 beta Antibody Peroxidase Conjugated

Application Note :

This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15 kDa in size corresponding to human MIP-1ß protein by western blotting in the appropriate cell lysate or extract. Immunology Research

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

HRP

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000

Western Blot Dilution :

1:500

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

CCL4

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

MIP-1 beta Antibody Peroxidase Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-1ß protein.

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Purity and Specificity :

Human MIP-1 beta Antibody Peroxidase Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-9 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

100 µL

Species Reactivity :

human

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store MIP-1 beta Antibody Peroxidase Conjugated at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-MIP-1 beta Antibody Peroxidase Conjugated, rabbit anti-MIP-1b Antibody HRP Conjugated, CCL4, C-C motif chemokine 4, Small-inducible cytokine A4, Macrophage inflammatory protein 1-beta, MIP-1-beta, ACT-2, T-cell activation protein 2, Protein H400, Lymphocyte activation gene 1 protein, LAG-1, HC21, G-26 T-lymphocyte-secreted protein, MIP-1ß

Target Species :

Human

Background :

MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94% - 98% identical and these proteins are all approximately 75% homologous to murine MIP1 beta. MIP1 beta also shares approximately 70% amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated. MIP-1 beta Antibody Peroxidase Conjugated is useful for researchers interested in Immunology Research.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Peroxidase (Horseradish)

UniProt :

P13236

NCBI :

NP_002975.1

more info or order :

Rockland Immunochemicals product webpage