product summary
Loading...
company name :
Rockland Immunochemicals
product type :
antibody
product name :
FIH Antibody
catalog :
209-301-F27
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
FIH 162c
application :
western blot, immunohistochemistry
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
209-301-F27
Name :
Anti-FIH (MOUSE) Monoclonal Antibody - 209-301-F27
Display Name :
FIH Antibody
Application Note :
Anti-FIH Antibody is recommended for use in WB and IHC (Paraffin embedded). Expect a band approximately ~45kDa corresponding to specific lysates. Specific conditions for reactivity should be optimized by the end user.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
FIH 162c
Concentration Value :
1mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100 µg
Default Unit :
µg
Immunohistochemistry Dilution :
User Optimized
Western Blot Dilution :
10ug/mL
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG1
Host Animal :
Mouse
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
FIH Antibody was produced in mice by repeated immunizations with full length human FIH expressed in E.coli BL21 (DE3) cells.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Purity and Specificity :
Anti-FIH Antibody was purified by Protein G chromatography. This monoclonal antibody is specific for human FIH protein. A BLAST analysis was used to suggest cross-reactivity with FIH from human based on 100% homology with the immunizing sequence. Cross-reactivity with FIH from other sources has not been determined. Oxidative Stress/ Cell Signaling research.
Species Reactivity :
Human
Stabilizer :
50% (v/v) Glycerol
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Subclass :
IgG1
Synonyms :
mouse anti-FIH Antibody, mouse anti-Factor inhibiting HIF1 antibody, Factor inhibiting HIF1 (hypoxia-inducible factor)
Target Species :
Human
Background :
FIH, Factor inhibiting HIF1 (hypoxia-inducible factor), is an asparaginyl hydroxylase. FIH in conjunction with VHL represses HIF-1 transcriptional activity by disrupting the interaction of HIF-1 with the transcriptional coactivators CBP/p300, and by recruiting histone deacetylases. FIH activity is inhibited during hypoxia. Recent studies show that low nuclear expression of FIH is a strong independent prognostic factor for a poor overall survival in clear cell renal cell cancinoma.
Immunogen Type :
Recombinant Protein
Low Endotoxin :
No
Sample Size :
No
Application Text :
Immunohistochemistry,Western Blot,
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
Primary Image Name :
FIH Immunohistochemistry
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.