CD74 Antibody | Rockland Immunochemicals 209-301-F12 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

CD74 Antibody

catalog :

209-301-F12

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PIN.1

reactivity :

human

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

209-301-F12

Name :

Anti-CD74 (MOUSE) Monoclonal Antibody - 209-301-F12

Display Name :

CD74 Antibody

Application Note :

Anti-CD74 Antibody is suitable for WB, IP, Flow Cytometry, FACS, and IHC. Expect a band approximately ~33-35kDa protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

PIN.1

Concentration Value :

1mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

Flow Cytometry Dilution :

User Optimized

Immunohistochemistry Dilution :

User Optimized

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

1 μg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

CD74

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

CD74 Antibody was produced in mice by repeated immunizations with a human CD74 invariant chain synthetic peptide.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.09% (w/v) Sodium Azide

Purity and Specificity :

Anti-CD74 Antibody was immunoglobulin purified. This monoclonal antibody is specific for human CD74. A BLAST analysis was used to suggest cross-reactivity with CD74 from human based on 100% homology with the immunizing sequence. Cross-reactivity with CD97 from other sources has not been determined. Cell Signaling research.

Species Reactivity :

Human

Stabilizer :

50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG

Synonyms :

mouse anti-CD74 antibody, mouse anti-Cluster of Differentiation 74 antibody, DHLAG, HLA DR gamma, HLADG, p33, p35, Protein 41 antibody, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain

Target Species :

Human

Background :

CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain. The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC); however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules. Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P04233

NCBI :

NP_001020329.1

Primary Image Name :

CD74 Western Blot

more info or order :

Rockland Immunochemicals product webpage