IL-6 Antibody | Rockland Immunochemicals 209-301-310 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

IL-6 Antibody

catalog :

209-301-310

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

33A12.G9

reactivity :

human

application :

western blot, ELISA, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

209-301-310

Name :

Anti-Human IL-6 (MOUSE) Monoclonal Antibody - 209-301-310

Display Name :

IL-6 Antibody

Application Note :

Anti-IL-6 antibody has been tested for use in ELISA, Flow Cytometry, and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

33A12.G9

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000

Flow Cytometry Dilution :

0.5 mg/mL

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

IL6

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This Protein A purified IL-6 monoclonal antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-6 produced in E.coli followed by hybridoma development.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This purified antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.

Species Reactivity :

Human

Storage :

Store IL 6 antibody at -20° C . For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1 kappa

Synonyms :

mouse Anti-IL-6 antibody, mouse anti-interleukin-6 antibody, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF

Target Species :

Human

Background :

IL-6 is a secreted cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Anti-IL-6 antibody antibody is ideal for investigators involved in Cancer, Neuroscience and Immunology research.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Yes

Sample Size :

Application Text :

ELISA,Flow Cytometry,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P05231

NCBI :

NP_000591.1

Primary Image Name :

Anti-Human IL-6 Antibody - Flow Cytometry

more info or order :

Rockland Immunochemicals product webpage