AKT2 Antibody
Rockland Immunochemicals
catalog: 600-401-425
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, flow cytometry

Western Blot of Rabbit Anti-AKT2 antibody. Lane 1: 0 min. Lane 2: 15 min 4OHT (tamoxifen). Lane 3: 30 min 4OHT (tamoxifen). Lane 4: 0 min lysate pretreated-for-15min-with 25uM LY294002. Lane 5: 15 min 4OHT (tamoxifen) lysate pretreated-for-15min-with 25uM LY294002. Lane 6: 30 min 4OHT (tamoxifen) lysate pretreated-for-15min-with 25uM LY294002. Load: 20mg/lane crude HEK293 lysate. Primary antibody: Anti-AKT2 Antibody at 1:1000 for 1h at room temperature. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Observed size: Endogenous Akt2 ~60kDa, myristoylated construct ~110kDa. Other band(s): This treatment has no effect on endogenous Akt2, but causes a band shift upwards in the MyrAkt2.
quantity: 100 µg
price:
to the supplier
AKT2 Antibody
Rockland Immunochemicals
catalog: 200-501-E71
rat monoclonal (16G11.E8)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry

Western Blot of Rat anti-AKT2 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT2 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rat secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT2. Other band(s): none.

Western Blot of Rat Anti-AKT2 antibody. Lane 1: C2C12. Lane 2: MEF#1. Lane 3: MEF#2. Lane 4: A549. Lane 5: Calu-1. Lane 6: PC3. Lane 7: HepG2. Lane 8: Jurkat. Lane 9: SKOV3. Lane 10: 293T. Load: 35 µg per lane. Primary antibody: AKT-2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Rat secondary antibody at 1:20,000 for 1 h at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT2.
quantity: 100 µg
price:
to the supplier
AKT2 Antibody
Rockland Immunochemicals
catalog: 200-501-J35
rat monoclonal (11F6.B2)
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry

Western Blot of Rat Anti-AKT2 antibody. Lane 1: LnCap lysate Lane 2: Jurkat lysate. Lane 3: MDA-MB 468 lysate. Load: 5 µg per lane. Primary antibody: AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Rat secondary antibody at 1:20,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT2.

Western Blot of Rat Anti-AKT2 antibody. Lane 1: LnCap lysate Lane 2: Jurkat lysate. Lane 3: MDA-MB 468 lysate. Load: 5 µg per lane. Primary antibody: AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Rat secondary antibody at 1:20,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT2.
quantity: 100 µg
price:
to the supplier
AKT2 Antibody
Rockland Immunochemicals
catalog: 200-301-BR8
mouse monoclonal (16C8.E4.G7.E9)
reactivity: human
application: western blot, ELISA
quantity: 100 µg
price:
to the supplier
AKT2 FITC Antibody
Rockland Immunochemicals
catalog: 200-502-E71
rat monoclonal (16G11.E8)
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, flow cytometry
quantity: 50 µg
price:
to the supplier
AKT2 FITC Antibody
Rockland Immunochemicals
catalog: 200-502-J35
rat monoclonal (11F6.B2)
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, flow cytometry
quantity: 50 µg
price:
to the supplier
AKT2 BIOTIN Antibody
Rockland Immunochemicals
catalog: 200-506-E71
rat monoclonal (16G11.E8)
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, flow cytometry
quantity: 50 µg
price:
to the supplier
AKT2 BIOTIN Antibody
Rockland Immunochemicals
catalog: 200-506-J35
rat monoclonal (11F6.B2)
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, flow cytometry
quantity: 50 µg
price:
to the supplier
AKT2 PE Antibody
Rockland Immunochemicals
catalog: 200-508-E71
rat monoclonal (16G11.E8)
reactivity: human
conjugate: RPE
application: western blot, ELISA, immunohistochemistry, flow cytometry
quantity: 50 µg
price:
to the supplier
AKT2 PE Antibody
Rockland Immunochemicals
catalog: 200-508-J35
rat monoclonal (11F6.B2)
reactivity: human
conjugate: RPE
application: western blot, ELISA, immunohistochemistry, flow cytometry
quantity: 50 µg
price:
to the supplier
AKT2 APC Antibody
Rockland Immunochemicals
catalog: 200-526-E71
rat monoclonal (16G11.E8)
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, flow cytometry
quantity: 50 µg
price:
to the supplier
AKT2 APC Antibody
Rockland Immunochemicals
catalog: 200-526-J35
rat monoclonal (11F6.B2)
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, flow cytometry
quantity: 50 µg
price:
to the supplier
AKT ISOFORM ANTIBODY SAMPLER KIT
Rockland Immunochemicals
catalog: K401
mouse monoclonal (various)
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry
quantity: 1 Kit
price:
to the supplier
AKT2 Western Chemiluminescent Blotting Kit
Rockland Immunochemicals
catalog: KCAE71
rat monoclonal (16G11.E8)
reactivity: human
application: western blot
quantity: 1 Kit
price:
to the supplier