domestic rabbit polyclonal
reactivity: mouse, rat
application: western blot, ELISA
reactivity: mouse, rat
application: western blot, ELISA
Western blot using Rockland's anti-Mouse MIP3a antibody shows detection of a band ~11 kDa in size corresponding to recombinant mouse MIP3a. Recombinant mouse MIP-3a was loaded on to an SDS-PAGE gel at 0.25 µg and after separation was transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reation with FemtoMax™ Chemiluminescent substrate (p/n FEMTOMAX-110). Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: mouse, rat
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
reactivity: mouse, rat
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
domestic rabbit polyclonal
reactivity: mouse, rat
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
reactivity: mouse, rat
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
rat monoclonal (4N5F7)
reactivity: mouse
application: western blot
reactivity: mouse
application: western blot
Western Blot of Mouse MIP 3? (RAT) antibody Lane 1: mouse recombinant MIP-3a Lane 2: human recombinant MIP-3a Primary antibody: MIP 3? antibody at 0.1 ug/ml for overnight at 4°C Secondary antibody: IRDye800™ goat anti-rat at 1:10,000 for 45 min at RT Block: 5% BLOTTO overnight at 4°C
quantity: 100 µg
price:
to the supplier
rat monoclonal (4N5F7)
reactivity: mouse
conjugate: biotin
application: western blot
reactivity: mouse
conjugate: biotin
application: western blot
Western Blot of Mouse MIP 3? (RAT) antibody Lane 1: mouse recombinant MIP-3a Lane 2: human recombinant MIP-3a Primary antibody: MIP 3? antibody at 0.1 ug/ml for overnight at 4°C Secondary antibody: IRDye800™ goat anti-rat at 1:10,000 for 45 min at RT Block: 5% BLOTTO overnight at 4°C
quantity: 100 µg
price:
to the supplier