domestic rabbit polyclonal
application: western blot, ELISA, immunohistochemistry
citations: 4
application: western blot, ELISA, immunohistochemistry
citations: 4
Western blotting using Rockland's Fluorescein conjugated anti-b-Galactosidase antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting (p/n MB-070). The membrane was probed with fluorescein conjugated anti-b-Galactosidase (p/n 200-4236) diluted to 1:10,000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc® Imaging System developed by BIO-RAD. Other detection systems will yield similar results
quantity: 100
price: https://www.rockland.com/search/?searchString=200-4136-0100
to the supplier
domestic rabbit polyclonal
reactivity:
application: western blot, ELISA, immunohistochemistry
citations: 3
reactivity:
application: western blot, ELISA, immunohistochemistry
citations: 3
Western blotting using Rockland's Fluorescein conjugated anti-b-Galactosidase antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of ?-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting (p/n MB-070). The membrane was probed with fluorescein conjugated anti-?-Galactosidase (p/n 200-4236) diluted to 1:10,000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc® Imaging System developed by BIO-RAD. Other detection systems will yield similar results.
quantity: 2 mL
price:
to the supplier
domestic rabbit polyclonal
conjugate: FITC
application: western blot
conjugate: FITC
application: western blot
Western blotting using Rockland's Fluorescein conjugated anti-ß-Galactosidase antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of ß-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting (p/n MB-070). The membrane was probed with fluorescein conjugated anti-b-Galactosidase (p/n 200-4236) diluted to 1:10,000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc® Imaging System developed by BIO-RAD. Other detection systems will yield similar results.
quantity: 10
price: 2,506.00 USD
to the supplier
domestic rabbit polyclonal
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
domestic rabbit polyclonal
conjugate: FITC
application: western blot
conjugate: FITC
application: western blot