This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Rockland Immunochemicals
product type :
antibody
product name :
CD20 Antibody
catalog :
200-C01-B40
quantity :
100 µL
price :
349.00 USD
clonality :
monoclonal
host :
rabbit
conjugate :
nonconjugated
clone name :
SP32
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, flow cytometry
product information
Catalog Number :
200-C01-B40
Name :
Anti-CD20 (RABBIT) Monoclonal Antibody - 200-C01-B40
Display Name :
CD20 Antibody
Application Note :
This monoclonal antibody is suitable for ELISA, immunohistochemistry, flow cytometry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 35 kDa in size corresponding to CD20 protein by western blotting in the appropriate cell lysate or extract. Ramos Cell lysate can be used as a positive control. CD20 is found in the cytoplasm and at the cell membrane. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Deparaffinize slides using xylene or xylene alternatives and graded alcohols. No special pretreatment of tissue is required for staining. Tonsil tissue or B cell lymphoma can be used as a positive control. For Flow cytometry use jurkat cell line as the positive control.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
SP32
Conjugation :
(None)
Size :
100 µL
Default Unit :
ul
ELISA Dilution :
1:10,000
Flow Cytometry Dilution :
1:100
Immunohistochemistry Dilution :
1:100
Western Blot Dilution :
1:200-1:500
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Gene Name :
MS4A1
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-CD20 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues at the carboxy terminus of human CD20 protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology.
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.1% (w/v) Sodium Azide
Purity and Specificity :
CD20 Antibody was purified from concentrated tissue culture supernate by Protein A/G chromatography. This antibody is specific for human CD20 protein. Cross-reactivity with CD20 protein from other sources has not been determined.
Species Reactivity :
Human
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store at 2-8°C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. Contains 1% BSA.
Subclass :
IgG
Synonyms :
CD20 membrane-spanning 4-domains subfamily A member 1 B-lymphocyte surface antigen B1 Leu-16 Bp35
Target Species :
Human
Background :
The MS4A1 gene encodes a member of the membrane-spanning 4A gene family termed the B-lymphocyte antigen CD20 (also known as CD20, membrane-spanning 4-domains subfamily A member 1, B-lymphocyte surface antigen B1, Leu-16 and Bp35). Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. The protein is expressed by B cells as a surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. Monoclonal antibodies against CD20 are used to treat B-cell non-Hodgkin's lymphoma (Rituximab, Britumomab and Tositumomab). Ideal for Cancer, Immunology and Stem Cell research.
General Reference :
Sar,A., Perizzolo,M., Stewart,D., Mansoor,A., Difrancesco,L.M. and Demetrick,D.J. (2009) Mutation or polymorphism of the CD20 gene is not associated with the response to R-CHOP in diffuse large B cell lymphoma patients. Leuk. Res. 33 (6), 792-797. Parolini,D., Meregalli,M., Belicchi,M., Razini,P., Lopa,R., DelCarlo,B., Farini,A., Maciotta,S., Bresolin,N., Porretti,L., Pellegrino,M. and Torrente,Y. (2009) CD20-related signaling pathway is differently activated in normal and dystrophic circulating CD133(+) stem cells. Cell. Mol. Life Sci. 66 (4), 697-710. Valentine,M.A., Licciardi,K.A., Clark,E.A., Krebs,E.G. and Meier,K.E. (1993) Insulin regulates serine/threonine phosphorylation in activated human B lymphocytes. J. Immunol. 150 (1), 96-105. Teeling J.L., Mackus W.J.M., Wiegman L.J.J.M., van den Brakel J.H.N., Beers S.A., French R.R., van Meerten T., Ebeling S., Vink T., Slootstra J.W., Parren P.W.H.I., Glennie M.J., van de Winkel J.G.J. (2006) The biological activity of human CD20 monoclonal antibodies is linked to unique epitopes on CD20. J. Immunol. 177:362-371.
Immunogen Type :
Peptide
Low Endotoxin :
No
Sample Size :
No
Target Domain of Reactivity :
C-Terminus
Created On :
12/15/2011 7:10 PM
Modified On :
3/27/2017 5:09 PM
Application Text :
ELISA,Flow Cytometry,Immunohistochemistry,Western Blot,
Approved for General Distributors :
12/1/2014
Discontinued Item :
No
Division :
OEM
Launch Date :
7/24/2009
Vendor :
Spring Bioscience
Status :
Active
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
2018 Price :
349.00 USD
UniProt :
P11836
NCBI :
AAH02807.1
Primary Image Name :
Anti-CD20 Monoclonal Antibody - Immunohistochemistry
company information
Rockland Immunochemicals
P.O. Box 326
Gilbertsville, PA 19525
Info@rockland-inc.com
www.rockland-inc.com
1-610-369-1008
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.