BAX Antibody | Rockland Immunochemicals 200-C01-B34 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

BAX Antibody

catalog :

200-C01-B34

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SP47

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-C01-B34

Name :

Anti-BAX (RABBIT) Monoclonal Antibody - 200-C01-B34

Display Name :

BAX Antibody

Application Note :

This monoclonal antibody is suitable for ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21 kDa in size corresponding to BAX protein by western blotting in the appropriate cell lysate or extract. BAX is found in normal breast tissue as a cytoplasmic protein and also at the cell membrane. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Deparaffinize slides using xylene or xylene alternatives and graded alcohols. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

SP47

Conjugation :

(None)

Size :

100 µL

Default Unit :

ELISA Dilution :

1:20,000

Flow Cytometry Dilution :

1:100

Immunohistochemistry Dilution :

1:50

Western Blot Dilution :

1:500 – 1:2,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

BAX

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human BAX protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.1% (w/v) Sodium Azide

Purity and Specificity :

This product was purified from concentrated tissue culture supernate by Protein A/G chromatography. This antibody is specific for human BAX protein. Cross-reactivity with BAX protein from other sources has not been determined.

Species Reactivity :

Human

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store at 2-8°C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. Contains 1% BSA.

Subclass :

IgG

Synonyms :

BAX Bcl-2-like protein 4 BCL2L4

Target Species :

Human

Background :

BAX, also known as Bcl-2-like protein 4 and BCL2L4 is known to accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. BAX induces the release of cytochrome c, activation of CASP3, and thereby apoptosis. BAX protein is expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histyocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. Isoform alpha is considered to be the 'canonical' sequence.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Flow Cytometry,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q07812

NCBI :

Q07812.1

Primary Image Name :

Anti-BAX Monoclonal Antibody - Immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage