This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Rockland Immunochemicals
product type :
antibody
product name :
Tau Antibody
catalog :
200-C01-B33
quantity :
100 µL
price :
349.00 USD
clonality :
monoclonal
host :
rabbit
conjugate :
nonconjugated
clone name :
SP70
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, flow cytometry
product information
Catalog Number :
200-C01-B33
Name :
Anti-Tau (RABBIT) Monoclonal Antibody - 200-C01-B33
Display Name :
Tau Antibody
Application Note :
This monoclonal antibody is suitable for ELISA, immunohistochemistry, flow cytometry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 45-68 kDa in size corresponding to Tau protein by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min. Use breast adenocarcinoma as positive control. For flow cytometry use SH-SY5Y Cell Line as positive control, location cytoplasm.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
SP70
Conjugation :
(None)
Size :
100 µL
Default Unit :
ul
ELISA Dilution :
1:20,000
Flow Cytometry Dilution :
1:100
Immunohistochemistry Dilution :
1:50
Western Blot Dilution :
1:500-1:2000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Gene Name :
MAPT
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to the N-terminus of human Tau protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology.
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.1% (w/v) Sodium Azide
Purity and Specificity :
This product was purified from concentrated tissue culture supernate by Protein A/G chromatography. This antibody is specific for human Tau protein. Cross-reactivity with Tau protein from other sources has not been determined.
Species Reactivity :
Human
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store at 2-8°C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. Contains 1% BSA.
Subclass :
IgG
Synonyms :
Microtubule-associated protein tau Neurofibrillary tangle protein Paired helical filament-tau
Target Species :
Human
Background :
The MAPT gene encodes the microtubule-associated protein Tau whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. Tau promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that Tau functions as a linker protein between both. Axonal polarity is predetermined by Tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of Tau.
General Reference :
Goedert M., Wischik C., Crowther R., Walker J., Klug A. (1988) Cloning and sequencing of the cDNA encoding a core protein of the paired helical filament of Alzheimer disease: identification as the microtubule-associated protein Tau. Proc. Natl. Acad. Sci. U.S.A. 85:4051-4055. Goedert M., Spillantini M.G., Potier M.-C., Ulrich J., Crowther R.A. (1989) Cloning and sequencing of the cDNA encoding an isoform of microtubule-associated protein Tau containing four tandem repeats: differential expression of Tau protein mRNAs in human brain. EMBO J. 8:393-399. Lee G., Neve R.L., Kosik K.S. (1989) The microtubule binding domain of Tau protein. Neuron 2:1615-1624. Goedert M., Spillantini M.G., Jakes R., Rutherford D., Crowther R.A. (1989) Multiple isoforms of human microtubule-associated protein Tau: sequences and localization in neurofibrillary tangles of Alzheimer's disease. Neuron 3:519-526. Andreadis A., Brown W.M., Kosik K.S. (1992) Structure and novel exons of the human Tau gene. Biochemistry 31:10626-10633.
Immunogen Type :
Peptide
Low Endotoxin :
No
Sample Size :
No
Target Domain of Reactivity :
N-Terminus
Created On :
12/15/2011 7:10 PM
Modified On :
7/20/2017 11:53 AM
Application Text :
ELISA,Flow Cytometry,Immunohistochemistry,Western Blot,
Approved for General Distributors :
12/1/2014
Discontinued Item :
No
Division :
OEM
Launch Date :
7/21/2009
Other :
User Optimized
Vendor :
Spring Bioscience
Status :
Active
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
2018 Price :
349.00 USD
UniProt :
P10636
NCBI :
P10636.5
Primary Image Name :
Anti-Tau Monoclonal Antibody - Immunohistochemistry
company information
Rockland Immunochemicals
P.O. Box 326
Gilbertsville, PA 19525
Info@rockland-inc.com
www.rockland-inc.com
1-610-369-1008
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.