product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
TCRbeta Fluorescein Antibody
catalog :
200-B02-N92
quantity :
500 µg
clonality :
monoclonal
host :
hamsters
conjugate :
FITC
clone name :
H57-597
reactivity :
mouse
application :
immunoprecipitation, flow cytometry
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
200-B02-N92
Name :
Anti-TCRbeta (Armenian Hamster) Fluorescein Conjugated Monoclonal Antibody - 200-B02-N92
Display Name :
TCRbeta Fluorescein Antibody
Application Note :
Anti-TCRbeta is useful for Immunofluorescence, Immunoprecipitation, and Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.
Buffer :
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
H57-597
Concentration Value :
0.5mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
FITC
Size :
500 µg
Default Unit :
µg
Flow Cytometry Dilution :
10 µL/10^6 cells (0.1 µg)
Fluorochrome Protein Value :
2-8
IF Microscopy Dilution :
User Optimized
Immunoprecipitation Dilution :
User Optimized
Expiration :
Expiration date is six (6) months from date of opening.
Format :
IgG
Gene Name :
Tcrb
Host Animal :
Hamster Armenian
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-TCRbeta Antibody (Monoclonal) was produced by repeated immunizations with TCRbeta antigen.
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.09% (w/v) Sodium Azide
Purity and Specificity :
Fluorescein conjugated TCRbeta Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse TCRbeta. Cross reactivity with TCRbeta from other sources has not been tested. Anti-TCRbeta is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.
Species Reactivity :
Mouse
Stabilizer :
0.1% Gelatin
Storage :
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
Subclass :
IgG
Synonyms :
Tib, TCRbeta, T cell receptor beta chain, Tcrb
Target Species :
Mouse
Background :
The H57-597 antibody is specific for the beta chain of the mouse T cell Receptor (TCR). This cell surface protein combines with a second protein chain (alpha chain) to form the alpha-beta TCR that is expressed by NK1.1+ thymocytes, NKT cells, and the majority of peripheral T cells. A small number of T cells may express an alternative heteromer of gamma and delta protein chains, known as the gamma-delta TCR. These receptors participate in a complex with CD3, and with the co-receptors CD4 or CD8, to recognize and respond to antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death.The H57-597 antibody is used as a phenotypic marker for T cells expressing the alpha-beta TCR. It is also widely used to cross-link surface TCR and thereby mimic TCR-mediated cell activation or induction of apoptosis. The antibody does not cross-react with cells expressing the gamma-delta TCR.
Low Endotoxin :
No
Sample Size :
No
Application Text :
Flow Cytometry,IF Microscopy,Immunoprecipitation,
Other :
Sufficient to run approximately 500 tests
Category :
Primary Antibodies
Conjugation Name :
Fluorescein (FITC)
Primary Image Name :
Flow Cytometry of anti-TCRbeta FITC - 200-B02-N92
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.