product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
CD80 Fluorescein Antibody
catalog :
200-B02-N89
quantity :
500 µg
clonality :
monoclonal
host :
hamsters
conjugate :
FITC
clone name :
16-10A1
reactivity :
mouse
application :
immunohistochemistry, immunoprecipitation, flow cytometry
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
200-B02-N89
Name :
Anti-CD80 (Armenian Hamster) Fluorescein Conjugated Monoclonal Antibody - 200-B02-N89
Display Name :
CD80 Fluorescein Antibody
Application Note :
Anti-CD80 is useful for Immunohistochemistry, Immunofluorescence, Immunoprecipitation, and Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.
Buffer :
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
16-10A1
Concentration Value :
0.5mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
FITC
Size :
500 µg
Default Unit :
µg
Flow Cytometry Dilution :
10 µL/10^6 cells (0.1 µg)
Fluorochrome Protein Value :
2-8
Immunohistochemistry Dilution :
User Optimized
IF Microscopy Dilution :
User Optimized
Immunoprecipitation Dilution :
User Optimized
Expiration :
Expiration date is six (6) months from date of opening.
Format :
IgG
Gene Name :
Cd80
Host Animal :
Hamster Armenian
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-CD80 Antibody (Monoclonal) was produced by repeated immunizations with CD80 antigen.
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.09% (w/v) Sodium Azide
Purity and Specificity :
Fluorescein conjugated CD80 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD80. Cross reactivity with CD80 from other sources has not been tested. Anti-CD80 is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.
Species Reactivity :
Mouse
Stabilizer :
0.1% Gelatin
Storage :
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
Subclass :
IgG
Synonyms :
T-lymphocyte activation antigen CD80, Activation B7-1 antigen, B7, CD80, Cd80
Target Species :
Mouse
Background :
Anti-CD80 antibody reacts with mouse CD80, also known as B7-1, a 55 kDa type I transmembrane protein ligand for CD152 (CTLA-4) and for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 also binds a second B7 ligand known as CD86 (B7-2). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 or CTLA-4 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.
Low Endotoxin :
No
Sample Size :
No
Application Text :
Flow Cytometry,Immunohistochemistry,IF Microscopy,Immunoprecipitation,
Other :
Sufficient to run approximately 500 tests
Category :
Primary Antibodies
Conjugation Name :
Fluorescein (FITC)
UniProt :
Q00609
NCBI :
NP_033985.3
Primary Image Name :
Flow Cytometry of anti-CD80 FITC - 200-B02-N89
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.