product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
CD3e Fluorescein Antibody
catalog :
200-B02-M69
quantity :
100 µg
clonality :
monoclonal
host :
hamsters
conjugate :
FITC
clone name :
145-2C11
reactivity :
mouse
application :
flow cytometry
product information
Catalog Number :
200-B02-M69
Name :
Anti-CD3 epsilon (ARMENIAN HAMSTER) Fluorescein Conjugated Monoclonal Antibody - 200-B02-M69
Display Name :
CD3e Fluorescein Antibody
Application Note :
Anti-CD3 is useful for Flow Cytometry (Cell Surface). Researchers should determine optimal titers for applications that are not stated.
Buffer :
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
145-2C11
Concentration Value :
0.5mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
FITC
Size :
100 µg
Default Unit :
µg
Flow Cytometry Dilution :
0.1-0.5 µg/10^6 cells
Fluorochrome Protein Value :
2-8
Expiration :
Expiration date is six (6) months from date of opening.
Format :
IgG
Gene Name :
Cd3e
Host Animal :
Hamster Armenian
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-CD3 Antibody (Monoclonal) was produced by repeated immunizations with H-2Kb specific cytotoxic T lymphocyte clone BM10-37.
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.09% (w/v) Sodium Azide
Purity and Specificity :
Fluorescein conjugated CD3 Monoclonal Antibody was Protein G Purified from tissue culture supernatant and is directed against mouse CD3. Reactivity is observed against mouse CD3. Cross reactivity with CD3 from other sources has not been tested.
Species Reactivity :
Mouse
Stabilizer :
0.1% Gelatin
Storage :
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
Subclass :
IgG
Synonyms :
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e
Target Species :
Mouse
Background :
CD3 is a multi-subunit complex consisting of 4 different invariable membrane proteins, CD3E, CD3D, CD3G and CD3-omega. These CD3 proteins are expressed on the surface of T-cells and Thymocytes during thymocyte development. They are non-covalently associated with the TCR heterodimer and form the functional TCR-CD3 antigen receptor complex of the T-lymphocyte. CD3 expression is significant during Intrathymic T-lymphocyte maturation, TCR signaling and Thymocyte differentiation. Stimulation of TCR-CD3 complex results in activation of various protein tyrosine kinases including ZAP-70 affecting multiple cellular responses including T-cell proliferation, clonal expansion, induction of tolerance and apoptosis. Mutations in the sub-unit chains of CD3 have been associated with various immunodeficiency syndromes including SCID.
Immunogen Type :
Other
Low Endotoxin :
No
Sample Size :
No
Application Text :
Flow Cytometry,
Other :
Sufficient to run approximately 500 tests
Category :
Primary Antibodies
Conjugation Name :
Fluorescein (FITC)
UniProt :
P22646
NCBI :
NP_031674.1
Primary Image Name :
Flow Cytometry - Hamster anti-MOUSE CD3 FITC
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.