EEF1A1 Antibody | Rockland Immunochemicals 200-901-MP6 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

EEF1A1 Antibody

catalog :

200-901-MP6

quantity :

100 µg

clonality :

polyclonal

host :

chicken

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Immunohistochemistry of Chicken Anti-EEF1A1 Antibody. Tissue: Human Kidney (renal tubular epithelium sections). Antigen Retrieval: HIER using Citrate buffer for 20mins. Fixative: none. Primary Antibody: Anti-EEF1A1 at 1:100 for 30 mins at RT. Secondary Antibody: Alpha Anti-Chicken HRP at 1:500 for 20mins at RT. Counter Stain: Hematoxylin. Predicted Localization: Intracellular, cytosol. Analysis Results: EEF1A1 shows weak cytoplasmic staining and more intense punctate staining. No membranous staining was observed.

product information

Catalog Number :

200-901-MP6

Name :

Anti-EEF1A1 (CHICKEN) Antibody - 200-901-MP6

Display Name :

EEF1A1 Antibody

Application Note :

Anti-EEF1A1 Antibody has been tested in ELISA, WB, and IHC. Expect a band at ~48-50 kDa in western blot using appropriate tissues and lysates. Positive control used: MCF-7 nuclear extract lysate in western blot and human kidney tissue in Immunohistochemistry.

Buffer :

0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0

Clonality :

Polyclonal

Concentration Value :

0.95 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000 - 1:50,000

Immunohistochemistry Dilution :

1:100

Western Blot Dilution :

5 µg/ml

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgY

Gene Name :

EEF1A1

Host Animal :

Chicken

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-EEF1A1 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of human EEF1A1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against human EEF1A1. This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to EEF1A1 human, mouse, rat, bovine, rabbit, cat, horse, Chinese hamster, Gallus gallus, Pan troglodytes, and Pongo abelii. May have cross-reactivity to EEF1A to Danio rerio; and eef1as to Xenopus laevis.

Species Reactivity :

Human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Chicken Anti-Eukaryotic Translation Elongation Factor 1 Alpha 1, Leukocyte Receptor Cluster (LRC) Member 7, Leukocyte Receptor Cluster Member 7, Eukaryotic Elongation Factor 1 A-1, Elongation Factor 1-Alpha 1, Elongation Factor Tu, EF-1-Alpha-1, EEF1A-1, EEF1A, LENG7, EF-Tu, EF1A, Eukaryotic Translation Elongation Factor 1 Alpha 1-Like 14, Glucocorticoid Receptor AF-1 Specific Elongation Factor, Translation Elongation Factor 1 Alpha 1-Like 14, Epididymis Secretory Sperm Binding Protein, Elongation Factor 1 Alpha Subunit, Prostate Tumor-Inducing Protein 1, Cervical Cancer Suppressor 3, CTCL Tumor Antigen, EF1a-Like Protein, HNGC:16303, GRAF-1EF, CCS-3, EE1A1, EEF-1, CCS3, PTI1

Target Species :

Human

Background :

EEF1A1 (Eukaryotic Translation Elongation Factor 1 Alpha 1) protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. Anti-EEF1A1 is useful for researchers interested in Eukaryotic Translation Elongation Factor 1 Alpha-1-Like 14, Cervical Cancer, and the Innate Immune system.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P68104

NCBI :

NP_001393.1

Primary Image Name :

Immunohistochemistry of Chicken Anti-EEF1A1 Antibody

more info or order :

Rockland Immunochemicals product webpage