XRCC1 Antibody | Rockland Immunochemicals 200-901-MG5S product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

XRCC1 Antibody

catalog :

200-901-MG5S

quantity :

25 µL

clonality :

polyclonal

host :

chicken

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Immunofluorescence of Chicken Anti-XRCC1 Antibody. Cells: U2OS cells. Fixative: 100% MeOH. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-XRCC1 at 10µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Chicken IgG DyLight™488 (p/n 603-141-126) at 5µg/mL for 60mins at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI, (B) Anti-XRCC1 and DyLight™488, (C) Merge A+B, (D) secondary only. Expected Localization: Nucleus and Nucleoplasm.

product information

Catalog Number :

200-901-MG5S

Name :

Anti-XRCC1 (CHICKEN) Antibody - 200-901-MG5S

Display Name :

XRCC1 Antibody

Application Note :

Anti-XRCC1 Antibody has been tested in ELISA, IF, and WB. Expect a band at ~69.5kDa in western blot using appropriate tissues and lysates. Positive control used: U2OS, MOLT4, Jurkat, and NIH-3T3 in WB. U2OS in Immunofluorescence.

Buffer :

0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0

Clonality :

Polyclonal

Concentration Value :

0.93 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

25 µL

Default Unit :

ELISA Dilution :

1:10,000-1:50,000

IF Microscopy Dilution :

10 µg/mL

Western Blot Dilution :

1:500-1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgY

Gene Name :

XRCC1

Host Animal :

Chicken

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-XRCC1 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a N-Terminal portion of human XRCC1 conjugated to Keyhole Limpet Hemocyanin (KLH). A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human and 91% to mouse, rat, and Chinese hamster.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This purified antibody is directed against human XRCC1. This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.

Species Reactivity :

human, mouse

Storage :

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Synonyms :

Chicken Anti-X-Ray Repair Cross Complementing 1 Antibody, X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 1, X-Ray Repair Cross-Complementing Protein 1, DNA Repair Protein XRCC1, SCAR26, RCC

Target Species :

Human

Background :

XRCC1 (X-Ray Repair Cross Complementing 1) is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Diseases associated with XRCC1 include Spinocerebellar Ataxia, Autosomal Recessive 26 and Gastric Cardia Carcinoma. Anti-XRCC1 Antibody is useful for researchers interested in Cancer research and DNA repair.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Yes

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P18887

NCBI :

NP_006288.2

Primary Image Name :

Immunofluorescence of Chicken Anti-XRCC1 Antibody

more info or order :

Rockland Immunochemicals product webpage