L1/ORF2 Antibody | Rockland Immunochemicals 200-901-B20 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

L1/ORF2 Antibody

catalog :

200-901-B20

quantity :

100

price :

https://www.rockland.com/search/?searchString=200-901-B20

clonality :

polyclonal

host :

chicken

conjugate :

nonconjugated

reactivity :

Human gammaherpesvirus 8

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-901-B20

Name :

Anti-L1/ORF2 (CHICKEN) Antibody - 200-901-B20

Display Name :

L1/ORF2 Antibody

Application Note :

Anti-L1/ORF2 Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 149 kDa in size corresponding to L1 protein by western blotting in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100

Default Unit :

µg

ELISA Dilution :

1:10,000

Western Blot Dilution :

1:2,000-1_10,000

Expiration :

Expiration date is one (1) year from date of receipt.

Format :

IgY

Gene Name :

ORF2

Host Animal :

Chicken

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

L1/ORF2 Antibody is directed against two regions within the endonuclease domain of L1 ORF2 protein. This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. Reactivity occurs against human L1/ORF2 protein and is useful in determining its presence in various assays. A BLAST analysis was used to suggest cross reactivity with L1/ORF2 proteins from chimpanzee sources based on 100% homology with the immunizing sequences, and from macaque, fruit fly, cattle, dog, opossum, and rat sources based on 69 - 88% homology with the immunizing sequences.

Species Reactivity :

Human; Chimpanzee

Tested Applications :

Dot Blot; ELISA; WB

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

chicken anti-L1/ORF2 Antibody, chicken anti-L1-ORF2 Antibody, L1 (LINE-1), Long INterspersed Element , retrotransposon ORF2

Target Species :

Human

Background :

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The L1 (LINE-1), or Long INterspersed Element , retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies. This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signaling. Both intact ORF1 and ORF2 are absolutely required for autonomous retro-transposition. ORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias. This antibody is intended for use in studying control of L1 retrotransposons.

Immunogen Type :

Conjugated Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2024 Price :

$475.00

UniProt :

O00370

NCBI :

AAD39215.1

Primary Image Name :

Anti-L1/ORF2 Antibody - Western Blot

more info or order :

Rockland Immunochemicals product webpage