HSF1 Antibody Allophycocyanin | Rockland Immunochemicals 200-526-W88 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

HSF1 Antibody Allophycocyanin

catalog :

200-526-W88

quantity :

100 µg

clonality :

monoclonal

host :

rat

conjugate :

APC

clone name :

10H4

application :

western blot, ELISA, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

200-526-W88

Name :

Anti-HSF1 (RAT) Monoclonal Antibody Allophycocyanin Conjugated - 200-526-W88

Display Name :

HSF1 Antibody Allophycocyanin

Application Note :

Anti-HSF1 APC Conjugated Antibody is suitable for Western Blots, Immunoprecipitation, ELISA, Gel Mobility Shift Assay and Immunocytochemistry. Expect a band approximately ~85kDa protein in unstressed cell lysates, and a 95 kDa protein in heat shocked cell lysates. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

10H4

Concentration Value :

1mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

APC

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000

Gel Shift Dilution :

User Optimized

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

Hsf1

Host Animal :

Rat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-HSF1 Antibody was produced by repeated immunization of rats with a purified recombinant mouse HSF1 protein, epitope mapping to amino acids 378-395.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.1% (w/v) Sodium Azide

Purity and Specificity :

Anti-HSF1 Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

Species Reactivity :

Human, Mouse, Rat, Bovine, Guinea Pig, Hamster, Monkey, Rabbit

Stabilizer :

50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. Dilute only prior to immediate use. This product is stable for several weeks at 4° C as an undiluted liquid. Centrifuge product if not completely clear after standing at room temperature. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing.

Subclass :

IgG1

Synonyms :

HSTF1, Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1

Target Species :

Mouse

Background :

HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11). Anti-HSF1 Antibody is ideal for research in Genetics, Transcription, Cell Signaling and pathways including ERK and MAPK.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Gel Shift,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Allophycocyanine

more info or order :

Rockland Immunochemicals product webpage